Detailed information of TA system TA216289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 141716..142327
• Replicon: plasmid p1
• Accession: NZ_CP083345
• Organism: Ensifer adhaerens strain W2-A

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: LAC78_RS36630
• Protein ID: WP_223730852.1
• Coordinates: 142046..142327 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LAC78_RS36625
• Protein ID: WP_223730851.1
• Coordinates: 141716..142015 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAC78_RS36600 (LAC78_36350)	138119..138952	+	834	WP_223730849.1	pyrroline-5-carboxylate reductase	-
LAC78_RS36605 (LAC78_36355)	139030..139224	+	195	WP_223730850.1	tautomerase family protein	-
LAC78_RS36610 (LAC78_36360)	140566..140927	+	362	Protein_136	DUF4112 domain-containing protein	-
LAC78_RS36615	141111..141191	-	81	WP_223731031.1	type II toxin-antitoxin system MqsA family antitoxin	-
LAC78_RS36620	141532..141696	-	165	WP_223731086.1	hypothetical protein	-
LAC78_RS36625 (LAC78_36375)	141716..142015	-	300	WP_223730851.1	HigA family addiction module antitoxin	Antitoxin
LAC78_RS36630 (LAC78_36380)	142046..142327	-	282	WP_223730852.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LAC78_RS36640 (LAC78_36390)	142928..143488	-	561	WP_223730854.1	hypothetical protein	-
LAC78_RS36645 (LAC78_36395)	144336..144811	+	476	Protein_142	DUF892 family protein	-
LAC78_RS36650 (LAC78_36400)	144862..145521	+	660	WP_223730855.1	hypothetical protein	-
LAC78_RS36655 (LAC78_36405)	146579..146797	-	219	Protein_144	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..735984	735984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-80)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10412.83 Da
• Isoelectric Point: 9.1204

>T216289 WP_223730852.1 NZ_CP083345:c142327-142046 [Ensifer adhaerens]
VIRSFKDAKTEAVARGKAPKGFPSDIVRSAVRKLTILNNAAALEDLRSPPGNRLEALKGNREGQHSIRINDQWRICFVWT
DAGIEDVEIVDYH

Nucleotide

• Length: 282 bp

>T216289 NZ_CP113005:c1665009-1664614 [Staphylococcus aureus]
ATGCAAAACATTAAATATTTAACTGAAAAACAAGTAATTGCCATTAATGTAAAAGCAATTCAAGAACTCTCACCTAAAGA
ACAAGTTGGTGTTAAAGTTCCAGAGGTTCTTAATGCTACTATTGAAGGAGTTAAACAATCATTTGGTGGAGTTGAACTAT
ATGAAACAATCGAGAGAAAAGCGGCTTTTATATACAGAAATATAGCTCAAAAACATGCATTCTTTAATGCGAATAAGAGA
ACAGCTTTTACATCTATGGTTATTTTCTTGAAGTTAAATAAGATTAATTTTAATTGTACTCAAGACGAAGCGGTACAATT
TACTTTAAAAGTGGTAGAGGATAAAACTCTTACACTTGAAGATATTGCAGATTGGATCAAAAGGCATTGTAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11312.13 Da
• Isoelectric Point: 6.2263

>AT216289 WP_223730851.1 NZ_CP083345:c142015-141716 [Ensifer adhaerens]
MTHILPPVHPGEILREEYLLPLNMSAGALAKKLNVPRTRIERIAAEQTPITTDTALRLAKYFRTTPEFWMNMQTSFNLKT
EEVALHSELELIPEIDRAA

Nucleotide

• Length: 300 bp

>AT216289 NZ_CP113005:c1665239-1665009 [Staphylococcus aureus]
ATGGTTACTACAAGAAAACTAAGAAGAGCTGGCAATAGTTCAGTTGTTTCTGTACCAACAGAAGTGATCGCAGCACTCGG
AGTTACAAATGGTGACAATCTTAAATTTAATATTGAAGATAATAAAGTAACCATTGAAAAGGAGGTAAACGAAGACGAAG
AGTTTTTCAAATTGTTGGATGAAACATTCACTGAGTATAACCAAGCTCTGAGAAAGTTGGTTGACATATAA