Detailed information of TA system TA216281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4170585..4171279
• Replicon: chromosome
• Accession: NZ_CP083274
• Organism: Escherichia coli strain Rosetta2-DE3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: K6U01_RS20065
• Protein ID: WP_001263493.1
• Coordinates: 4170881..4171279 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: K6U01_RS20060
• Protein ID: WP_000554757.1
• Coordinates: 4170585..4170878 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6U01_RS20040 (4166225)	4166225..4166722	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
K6U01_RS20045 (4166938)	4166938..4168650	-	1713	Protein_3947	flagellar biosynthesis protein FlhA	-
K6U01_RS20050 (4168622)	4168622..4169407	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
K6U01_RS20055 (4169478)	4169478..4170533	+	1056	WP_001226164.1	DNA polymerase IV	-
K6U01_RS20060 (4170585)	4170585..4170878	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
K6U01_RS20065 (4170881)	4170881..4171279	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
K6U01_RS20070 (4171289)	4171289..4171741	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
K6U01_RS20075 (4171987)	4171987..4172190	+	204	Protein_3953	RtcB family protein	-
K6U01_RS20080 (4172189)	4172189..4172710	+	522	Protein_3954	peptide chain release factor H	-
K6U01_RS20085 (4172767)	4172767..4174224	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
K6U01_RS20090 (4174485)	4174485..4174943	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (4175539)	4175539..4175619	+	81	NuclAT_13	-	-
- (4175539)	4175539..4175619	+	81	NuclAT_13	-	-
- (4175539)	4175539..4175619	+	81	NuclAT_13	-	-
- (4175539)	4175539..4175619	+	81	NuclAT_13	-	-
K6U01_RS20095 (4175035)	4175035..4176279	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	4156046..4172710	16664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T216281 WP_001263493.1 NZ_CP083274:4170881-4171279 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T216281 NZ_CP112997:3725502-3725759 [Mycobacterium tuberculosis variant bovis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT216281 WP_000554757.1 NZ_CP083274:4170585-4170878 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT216281 NZ_CP112997:3725230-3725505 [Mycobacterium tuberculosis variant bovis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6