Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4164549..4165090 Replicon chromosome
Accession NZ_CP083274
Organism Escherichia coli strain Rosetta2-DE3

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag K6U01_RS20025 Protein ID WP_000615981.1
Coordinates 4164549..4164827 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag K6U01_RS20030 Protein ID WP_000729704.1
Coordinates 4164830..4165090 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6U01_RS20010 (4162131) 4162131..4162709 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
K6U01_RS20015 (4162915) 4162915..4163682 + 768 WP_000333379.1 class II glutamine amidotransferase -
K6U01_RS20020 (4163653) 4163653..4164393 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K6U01_RS20025 (4164549) 4164549..4164827 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K6U01_RS20030 (4164830) 4164830..4165090 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K6U01_RS20035 (4165276) 4165276..4166049 + 774 WP_001353765.1 C40 family peptidase -
K6U01_RS20040 (4166225) 4166225..4166722 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
K6U01_RS20045 (4166938) 4166938..4168650 - 1713 Protein_3947 flagellar biosynthesis protein FlhA -
K6U01_RS20050 (4168622) 4168622..4169407 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4156046..4172710 16664


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T216280 WP_000615981.1 NZ_CP083274:c4164827-4164549 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T216280 NZ_CP083274:c4164827-4164549 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT216280 WP_000729704.1 NZ_CP083274:c4165090-4164830 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT216280 NZ_CP083274:c4165090-4164830 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References