Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2411567..2412294 Replicon chromosome
Accession NZ_CP083274
Organism Escherichia coli strain Rosetta2-DE3

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag K6U01_RS11580 Protein ID WP_000550189.1
Coordinates 2411567..2411881 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K6U01_RS11585 Protein ID WP_000560266.1
Coordinates 2411878..2412294 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6U01_RS11560 (2407725) 2407725..2408711 - 987 WP_012775974.1 Gfo/Idh/MocA family oxidoreductase -
K6U01_RS11565 (2408790) 2408790..2409482 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
K6U01_RS11570 (2409559) 2409559..2410062 - 504 WP_001300832.1 M48 family metallopeptidase -
K6U01_RS11575 (2410147) 2410147..2411283 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
K6U01_RS11580 (2411567) 2411567..2411881 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
K6U01_RS11585 (2411878) 2411878..2412294 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
K6U01_RS11590 (2412339) 2412339..2414357 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
K6U01_RS11600 (2415158) 2415158..2416096 + 939 WP_000351315.1 glyoxylate/hydroxypyruvate reductase GhrA -
K6U01_RS11605 (2416151) 2416151..2416888 + 738 WP_000283664.1 zinc-binding phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T216272 WP_000550189.1 NZ_CP083274:2411567-2411881 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T216272 NZ_CP112997:c3030028-3029612 [Mycobacterium tuberculosis variant bovis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT216272 WP_000560266.1 NZ_CP083274:2411878-2412294 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT216272 NZ_CP112997:c3030291-3030025 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References