Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4219161..4219702 Replicon chromosome
Accession NZ_CP083273
Organism Escherichia coli strain BL21-RIPL

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag K9U36_RS20430 Protein ID WP_000615981.1
Coordinates 4219161..4219439 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag K9U36_RS20435 Protein ID WP_000729704.1
Coordinates 4219442..4219702 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U36_RS20415 (4216743) 4216743..4217321 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
K9U36_RS20420 (4217527) 4217527..4218294 + 768 WP_000333379.1 class II glutamine amidotransferase -
K9U36_RS20425 (4218265) 4218265..4219005 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K9U36_RS20430 (4219161) 4219161..4219439 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K9U36_RS20435 (4219442) 4219442..4219702 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K9U36_RS20440 (4219888) 4219888..4220661 + 774 WP_001353765.1 C40 family peptidase -
K9U36_RS20445 (4220837) 4220837..4221334 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
K9U36_RS20450 (4221550) 4221550..4223262 - 1713 Protein_4026 flagellar biosynthesis protein FlhA -
K9U36_RS20455 (4223234) 4223234..4224019 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4210658..4227322 16664


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T216255 WP_000615981.1 NZ_CP083273:c4219439-4219161 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T216255 NZ_CP112997:1956076-1956324 [Mycobacterium tuberculosis variant bovis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT216255 WP_000729704.1 NZ_CP083273:c4219702-4219442 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT216255 NZ_CP112997:1955864-1956076 [Mycobacterium tuberculosis variant bovis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References