Detailed information of TA system TA216231

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62560..62981
• Replicon: plasmid p1
• Accession: NZ_CP083264
• Organism: Escherichia coli strain JNU

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K6T58_RS23850
• Protein ID: WP_096937776.1
• Coordinates: 62856..62981 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	62560..62758 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6T58_RS23820 (58490)	58490..59029	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
K6T58_RS23825 (59086)	59086..59319	+	234	WP_000005990.1	DUF905 family protein	-
K6T58_RS23830 (59385)	59385..61343	+	1959	WP_000117171.1	ParB/RepB/Spo0J family partition protein	-
K6T58_RS23835 (61398)	61398..61832	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
K6T58_RS23840 (61829)	61829..62591	+	763	Protein_57	plasmid SOS inhibition protein A	-
- (62560)	62560..62758	+	199	NuclAT_0	-	Antitoxin
- (62560)	62560..62758	+	199	NuclAT_0	-	Antitoxin
- (62560)	62560..62758	+	199	NuclAT_0	-	Antitoxin
- (62560)	62560..62758	+	199	NuclAT_0	-	Antitoxin
K6T58_RS23845 (62765)	62765..62914	+	150	Protein_58	DUF5431 family protein	-
K6T58_RS23850 (62856)	62856..62981	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
K6T58_RS23855 (63282)	63282..63578	-	297	Protein_60	hypothetical protein	-
K6T58_RS23860 (63648)	63648..63854	+	207	WP_000547963.1	hypothetical protein	-
K6T58_RS23865 (63879)	63879..64166	+	288	WP_000107542.1	hypothetical protein	-
K6T58_RS23870 (64285)	64285..65106	+	822	WP_001234474.1	DUF932 domain-containing protein	-
K6T58_RS23875 (65401)	65401..65991	-	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
K6T58_RS23880 (66324)	66324..66707	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K6T58_RS23885 (66894)	66894..67583	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..102134	102134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T216231 WP_096937776.1 NZ_CP083264:62856-62981 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T216231 NZ_CP112997:c443921-443328 [Mycobacterium tuberculosis variant bovis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT216231 NZ_CP083264:62560-62758 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin