Detailed information of TA system TA216223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2924964..2925566
• Replicon: chromosome
• Accession: NZ_CP083263
• Organism: Escherichia coli strain JNU

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: K6T58_RS14205
• Protein ID: WP_000897305.1
• Coordinates: 2925255..2925566 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6T58_RS14200
• Protein ID: WP_000356397.1
• Coordinates: 2924964..2925254 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6T58_RS14175 (2920511)	2920511..2921146	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
K6T58_RS14180 (2921143)	2921143..2922072	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
K6T58_RS14185 (2922471)	2922471..2923451	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
K6T58_RS14190 (2923680)	2923680..2923922	-	243	WP_001086388.1	protein YiiF	-
K6T58_RS14195 (2924141)	2924141..2924359	-	219	WP_001295676.1	CopG family transcriptional regulator	-
K6T58_RS14200 (2924964)	2924964..2925254	-	291	WP_000356397.1	NadS family protein	Antitoxin
K6T58_RS14205 (2925255)	2925255..2925566	-	312	WP_000897305.1	hypothetical protein	Toxin
K6T58_RS14210 (2925795)	2925795..2926703	+	909	WP_001162704.1	alpha/beta hydrolase	-
K6T58_RS14215 (2926767)	2926767..2927708	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
K6T58_RS14220 (2927753)	2927753..2928190	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
K6T58_RS14225 (2928187)	2928187..2929059	-	873	WP_000920762.1	virulence factor BrkB family protein	-
K6T58_RS14230 (2929053)	2929053..2929652	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2922471..2923451	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T216223 WP_000897305.1 NZ_CP083263:c2925566-2925255 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T216223 NZ_CP112996:3512482-3512826 [Mycobacterium tuberculosis variant bovis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT216223 WP_000356397.1 NZ_CP083263:c2925254-2924964 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT216223 NZ_CP112996:3512823-3513152 [Mycobacterium tuberculosis variant bovis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7