Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2018964..2019505 Replicon chromosome
Accession NZ_CP083263
Organism Escherichia coli strain JNU

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag K6T58_RS09895 Protein ID WP_000615976.1
Coordinates 2019227..2019505 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag K6T58_RS09890 Protein ID WP_000729704.1
Coordinates 2018964..2019224 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6T58_RS09865 (2014747) 2014747..2015532 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
K6T58_RS09870 (2015504) 2015504..2017216 + 1713 Protein_1933 flagellar biosynthesis protein FlhA -
K6T58_RS09875 (2017321) 2017321..2017599 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
K6T58_RS09880 (2017592) 2017592..2017948 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
K6T58_RS09885 (2018005) 2018005..2018778 - 774 WP_000543897.1 C40 family peptidase -
K6T58_RS09890 (2018964) 2018964..2019224 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K6T58_RS09895 (2019227) 2019227..2019505 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K6T58_RS09900 (2019661) 2019661..2020401 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K6T58_RS09905 (2020372) 2020372..2021139 - 768 WP_000333380.1 class II glutamine amidotransferase -
K6T58_RS09910 (2021345) 2021345..2021923 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T216220 WP_000615976.1 NZ_CP083263:2019227-2019505 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T216220 NZ_CP112996:c3097661-3097269 [Mycobacterium tuberculosis variant bovis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT216220 WP_000729704.1 NZ_CP083263:2018964-2019224 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT216220 NZ_CP112996:c3097873-3097658 [Mycobacterium tuberculosis variant bovis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References