Detailed information of TA system TA216192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 143636..144242
• Replicon: plasmid pB
• Accession: NZ_CP083241
• Organism: Ancylobacter polymorphus strain ZM13

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: K9D25_RS23020
• Protein ID: WP_244451148.1
• Coordinates: 143636..143917 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K9D25_RS23025
• Protein ID: WP_244451149.1
• Coordinates: 143931..144242 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9D25_RS22985 (K9D25_22985)	139460..139933	+	474	WP_244451141.1	hypothetical protein	-
K9D25_RS22990 (K9D25_22990)	140147..140407	-	261	WP_244451142.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K9D25_RS22995 (K9D25_22995)	140473..141189	-	717	WP_244451143.1	metallophosphoesterase	-
K9D25_RS23000 (K9D25_23000)	141252..141785	-	534	WP_244451144.1	3'-5' exonuclease	-
K9D25_RS23005 (K9D25_23005)	141821..142138	-	318	WP_244451145.1	WGR domain-containing protein	-
K9D25_RS23010 (K9D25_23010)	142503..142790	+	288	WP_244451146.1	hypothetical protein	-
K9D25_RS23015 (K9D25_23015)	142783..143493	+	711	WP_244451147.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
K9D25_RS23020 (K9D25_23020)	143636..143917	+	282	WP_244451148.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9D25_RS23025 (K9D25_23025)	143931..144242	+	312	WP_244451149.1	HigA family addiction module antitoxin	Antitoxin
K9D25_RS23030 (K9D25_23030)	144458..145219	-	762	WP_244451150.1	ABC transporter ATP-binding protein	-
K9D25_RS23035 (K9D25_23035)	145216..146274	-	1059	WP_244451319.1	iron ABC transporter permease	-
K9D25_RS23040 (K9D25_23040)	146283..147377	-	1095	WP_244451151.1	siderophore-interacting protein	-
K9D25_RS23045 (K9D25_23045)	147401..148519	-	1119	WP_244451152.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..232090	232090

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (7-80)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10251.64 Da
• Isoelectric Point: 7.2788

>T216192 WP_244451148.1 NZ_CP083241:143636-143917 [Ancylobacter polymorphus]
MIVSTKGKFVEAVIAGKAPKGFPADILRSAERRLRAVDAAVVLDDLRSPPGNHLEALSGDRAGQHSIRINDQWRICFRWT
EAGPADVEITDYH

Nucleotide

• Length: 282 bp

>T216192 NZ_CP112996:1385858-1386289 [Mycobacterium tuberculosis variant bovis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11484.22 Da
• Isoelectric Point: 5.8525

>AT216192 WP_244451149.1 NZ_CP083241:143931-144242 [Ancylobacter polymorphus]
MAVNFARPMHPGEFLREEYLVPLDMSPGRLAKALGLPRTRIERIAREEIGISTDTALRLARFFSTTPGFWLNLQQAYDVE
TEAPKLAADIARIEPYAPVGQAA

Nucleotide

• Length: 312 bp

>AT216192 NZ_CP112996:1385601-1385861 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA