Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1521805..1522367 Replicon chromosome
Accession NC_008253
Organism Escherichia coli 536

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag ECP_RS07530 Protein ID WP_000605675.1
Coordinates 1522089..1522367 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag ECP_RS07525 Protein ID WP_000781370.1
Coordinates 1521805..1522089 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECP_RS07510 1516986..1520033 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
ECP_RS07515 1520046..1520930 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
ECP_RS07520 1520923..1521576 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
ECP_RS07525 1521805..1522089 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
ECP_RS07530 1522089..1522367 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECP_RS07535 1522553..1523563 - 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
ECP_RS07540 1523697..1525394 - 1698 WP_000433462.1 malate dehydrogenase -
ECP_RS07545 1525551..1525688 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
ECP_RS07550 1525790..1526005 - 216 WP_000495766.1 biofilm-dependent modulation protein -
ECP_RS07555 1526350..1526781 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T21617 WP_000605675.1 NC_008253:c1522367-1522089 [Escherichia coli 536]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T21617 NC_008253:c1522367-1522089 [Escherichia coli 536]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT21617 WP_000781370.1 NC_008253:c1522089-1521805 [Escherichia coli 536]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT21617 NC_008253:c1522089-1521805 [Escherichia coli 536]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References