Detailed information of TA system TA021613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 283329..284023
• Replicon: chromosome
• Accession: NC_008253
• Organism: Escherichia coli 536

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: ECP_RS01325
• Protein ID: WP_001263500.1
• Coordinates: 283625..284023 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: ECP_RS01320
• Protein ID: WP_000554758.1
• Coordinates: 283329..283622 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECP_RS01300	278865..279362	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
ECP_RS26650	279445..279654	-	210	WP_011579064.1	hypothetical protein	-
ECP_RS01305	279682..281394	-	1713	Protein_249	flagellar biosynthesis protein FlhA	-
ECP_RS01310	281366..282151	+	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
ECP_RS01315	282222..283277	+	1056	WP_001226168.1	DNA polymerase IV	-
ECP_RS01320	283329..283622	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ECP_RS01325	283625..284023	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ECP_RS01330	284033..284485	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
ECP_RS01335	284675..285814	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
ECP_RS01340	285811..286425	+	615	WP_000602124.1	peptide chain release factor H	-
ECP_RS01345	286482..287939	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
ECP_RS01350	288200..288658	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T21613 WP_001263500.1 NC_008253:283625-284023 [Escherichia coli 536]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T21613 NC_008253:283625-284023 [Escherichia coli 536]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT21613 WP_000554758.1 NC_008253:283329-283622 [Escherichia coli 536]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT21613 NC_008253:283329-283622 [Escherichia coli 536]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3