Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3956137..3956734 Replicon chromosome
Accession NZ_CP083066
Organism Klebsiella pneumoniae strain 1613015451

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9F39_RS19590 Protein ID WP_004142563.1
Coordinates 3956417..3956734 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9F39_RS19585 Protein ID WP_004142561.1
Coordinates 3956137..3956424 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F39_RS19555 (3952217) 3952217..3952465 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K9F39_RS19560 (3952483) 3952483..3952824 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K9F39_RS19565 (3952855) 3952855..3953970 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
K9F39_RS19570 (3954150) 3954150..3954731 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
K9F39_RS19575 (3954731) 3954731..3955099 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
K9F39_RS19580 (3955219) 3955219..3955872 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
K9F39_RS19585 (3956137) 3956137..3956424 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9F39_RS19590 (3956417) 3956417..3956734 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9F39_RS19595 (3956919) 3956919..3957962 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
K9F39_RS19600 (3958629) 3958629..3959495 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
K9F39_RS19605 (3959604) 3959604..3961031 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T216095 WP_004142563.1 NZ_CP083066:c3956734-3956417 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T216095 NZ_CP112873:c1351166-1350420 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT216095 WP_004142561.1 NZ_CP083066:c3956424-3956137 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT216095 NZ_CP112873:c1350420-1350250 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References