Detailed information of TA system TA216040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3847504..3848101
• Replicon: chromosome
• Accession: NZ_CP083051
• Organism: Klebsiella pneumoniae strain 0213293972

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: K9F37_RS19180
• Protein ID: WP_004893639.1
• Coordinates: 3847784..3848101 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: K9F37_RS19175
• Protein ID: WP_004142561.1
• Coordinates: 3847504..3847791 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F37_RS19145 (3843711)	3843711..3843959	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
K9F37_RS19150 (3843977)	3843977..3844318	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
K9F37_RS19155 (3844349)	3844349..3845464	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
K9F37_RS19160 (3845644)	3845644..3846225	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
K9F37_RS19165 (3846225)	3846225..3846593	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
K9F37_RS19170 (3846713)	3846713..3847366	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
K9F37_RS19175 (3847504)	3847504..3847791	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
K9F37_RS19180 (3847784)	3847784..3848101	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9F37_RS19185 (3848286)	3848286..3849329	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
K9F37_RS19190 (3849999)	3849999..3850865	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
K9F37_RS19195 (3850974)	3850974..3852401	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T216040 WP_004893639.1 NZ_CP083051:c3848101-3847784 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T216040 NZ_CP111114:c320061-319747 [Pseudomonas koreensis]
ATGATTTTTATTGAGACTCGGATCTTTACCCGTCGAGTAAAAGAGCTGCTGGATGATGACACGTACGCCGCTTTTCAGAA
GCAACTGGTTGTAAGCCCTTCGATTGGCGATGTAATTGAAGGCACAGGCGGGATTCGCAAAACCCGAATCGCAGCGAAAG
GACACGGCAAGCGAGGCGGAGCACGTGTTATTTACTACCACTTCGTTTCGGCGTCACAAATCGGGTTATTGATGATCTAC
CCTAAAAATGAACAACACGATCTGTCCAGCGATGAGCGTAAAGCACTCAAGGTCTTGATCGAAAAATGGAGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT216040 WP_004142561.1 NZ_CP083051:c3847791-3847504 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT216040 NZ_CP111114:c319744-319457 [Pseudomonas koreensis]
ATGAGCAAGTTTTTTGATGAGCTGATGGAAAGTGTGCAAGAGATGGACGAGATCGTACGAGGCGAGCGCACACCTTCTCG
CGAATTCCACGTTGATGCATTGCAGGTCAAAGAAATTCGTAAAGCTACCGGTTTGACCCAGACCAAATTTGCGGCACTGA
TCGACGTCCAACTCGGCACTCTACGAAATTGGGAACAGGGCCGTCGCGAGCCAACCGGGCCGGCAAAAGCTTTGCTCAAG
GCCATTCATAACGACCCGGTTCATGTTTTGAATGCATTGGCTCATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1