Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4053379..4053976 Replicon chromosome
Accession NZ_CP083047
Organism Klebsiella pneumoniae strain 400195-1-18

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9F24_RS19885 Protein ID WP_004142563.1
Coordinates 4053659..4053976 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9F24_RS19880 Protein ID WP_004142561.1
Coordinates 4053379..4053666 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F24_RS19850 (4049459) 4049459..4049707 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K9F24_RS19855 (4049725) 4049725..4050066 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K9F24_RS19860 (4050097) 4050097..4051212 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
K9F24_RS19865 (4051392) 4051392..4051973 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
K9F24_RS19870 (4051973) 4051973..4052341 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
K9F24_RS19875 (4052461) 4052461..4053111 + 651 Protein_3909 oxygen-insensitive NAD(P)H nitroreductase -
K9F24_RS19880 (4053379) 4053379..4053666 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9F24_RS19885 (4053659) 4053659..4053976 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9F24_RS19890 (4054161) 4054161..4055204 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
K9F24_RS19895 (4055871) 4055871..4056737 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
K9F24_RS19900 (4056846) 4056846..4058273 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T216017 WP_004142563.1 NZ_CP083047:c4053976-4053659 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T216017 NZ_CP111032:c5017423-5017058 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT216017 WP_004142561.1 NZ_CP083047:c4053666-4053379 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT216017 NZ_CP111032:c5017065-5016742 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
ATTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCATCTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References