Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4053263..4053860 Replicon chromosome
Accession NZ_CP083043
Organism Klebsiella pneumoniae strain 400195-2-18

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9F25_RS19885 Protein ID WP_004142563.1
Coordinates 4053543..4053860 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9F25_RS19880 Protein ID WP_004142561.1
Coordinates 4053263..4053550 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F25_RS19850 (4049343) 4049343..4049591 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K9F25_RS19855 (4049609) 4049609..4049950 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K9F25_RS19860 (4049981) 4049981..4051096 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
K9F25_RS19865 (4051276) 4051276..4051857 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
K9F25_RS19870 (4051857) 4051857..4052225 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
K9F25_RS19875 (4052345) 4052345..4052995 + 651 Protein_3909 oxygen-insensitive NAD(P)H nitroreductase -
K9F25_RS19880 (4053263) 4053263..4053550 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9F25_RS19885 (4053543) 4053543..4053860 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9F25_RS19890 (4054045) 4054045..4055088 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
K9F25_RS19895 (4055755) 4055755..4056621 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
K9F25_RS19900 (4056730) 4056730..4058157 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T215996 WP_004142563.1 NZ_CP083043:c4053860-4053543 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T215996 NZ_CP111029:c2605950-2605847 [Salmonella sp. 2018103]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT215996 WP_004142561.1 NZ_CP083043:c4053550-4053263 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT215996 NZ_CP111029:2605845-2605988 [Salmonella sp. 2018103]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References