Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3997352..3997949 Replicon chromosome
Accession NZ_CP083036
Organism Klebsiella pneumoniae strain 4906.28

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9F35_RS19780 Protein ID WP_004142563.1
Coordinates 3997632..3997949 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9F35_RS19775 Protein ID WP_004142561.1
Coordinates 3997352..3997639 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F35_RS19745 (3993433) 3993433..3993681 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K9F35_RS19750 (3993699) 3993699..3994040 - 342 WP_064181824.1 RamA family antibiotic efflux transcriptional regulator -
K9F35_RS19755 (3994071) 3994071..3995186 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
K9F35_RS19760 (3995365) 3995365..3995946 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
K9F35_RS19765 (3995946) 3995946..3996314 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
K9F35_RS19770 (3996434) 3996434..3997087 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
K9F35_RS19775 (3997352) 3997352..3997639 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9F35_RS19780 (3997632) 3997632..3997949 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9F35_RS19785 (3998134) 3998134..3999177 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
K9F35_RS19790 (3999844) 3999844..4000710 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
K9F35_RS19795 (4000819) 4000819..4002246 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T215979 WP_004142563.1 NZ_CP083036:c3997949-3997632 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T215979 NZ_CP111008:2473065-2473172 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT215979 WP_004142561.1 NZ_CP083036:c3997639-3997352 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT215979 NZ_CP111008:c2473015-2472952 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References