Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3974999..3975596 Replicon chromosome
Accession NZ_CP083026
Organism Klebsiella pneumoniae strain 6604.68

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9F26_RS19525 Protein ID WP_004142563.1
Coordinates 3975279..3975596 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9F26_RS19520 Protein ID WP_004142561.1
Coordinates 3974999..3975286 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F26_RS19490 (3971080) 3971080..3971328 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K9F26_RS19495 (3971346) 3971346..3971687 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K9F26_RS19500 (3971718) 3971718..3972833 - 1116 Protein_3837 MBL fold metallo-hydrolase -
K9F26_RS19505 (3973012) 3973012..3973593 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
K9F26_RS19510 (3973593) 3973593..3973961 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
K9F26_RS19515 (3974081) 3974081..3974734 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
K9F26_RS19520 (3974999) 3974999..3975286 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9F26_RS19525 (3975279) 3975279..3975596 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9F26_RS19530 (3975781) 3975781..3976824 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
K9F26_RS19535 (3977491) 3977491..3978357 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
K9F26_RS19540 (3978466) 3978466..3979893 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T215940 WP_004142563.1 NZ_CP083026:c3975596-3975279 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T215940 NZ_CP111004:c964625-964296 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT215940 WP_004142561.1 NZ_CP083026:c3975286-3974999 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT215940 NZ_CP111004:c964963-964646 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References