Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3958685..3959282 Replicon chromosome
Accession NZ_CP083016
Organism Klebsiella pneumoniae strain 6709.15-I

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9F34_RS19425 Protein ID WP_004142563.1
Coordinates 3958965..3959282 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9F34_RS19420 Protein ID WP_004142561.1
Coordinates 3958685..3958972 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F34_RS19390 (3954766) 3954766..3955014 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K9F34_RS19395 (3955032) 3955032..3955373 - 342 WP_231535943.1 RamA family antibiotic efflux transcriptional regulator -
K9F34_RS19400 (3955404) 3955404..3956519 - 1116 Protein_3817 MBL fold metallo-hydrolase -
K9F34_RS19405 (3956698) 3956698..3957279 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
K9F34_RS19410 (3957279) 3957279..3957647 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
K9F34_RS19415 (3957767) 3957767..3958420 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
K9F34_RS19420 (3958685) 3958685..3958972 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9F34_RS19425 (3958965) 3958965..3959282 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9F34_RS19430 (3959467) 3959467..3960510 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
K9F34_RS19435 (3961177) 3961177..3962043 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
K9F34_RS19440 (3962152) 3962152..3963579 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T215904 WP_004142563.1 NZ_CP083016:c3959282-3958965 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T215904 NZ_CP110998:c103300-102884 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT215904 WP_004142561.1 NZ_CP083016:c3958972-3958685 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT215904 NZ_CP110998:c103527-103297 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References