Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3970858..3971455 Replicon chromosome
Accession NZ_CP083011
Organism Klebsiella pneumoniae strain 6710.71

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9F28_RS19545 Protein ID WP_004142563.1
Coordinates 3971138..3971455 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9F28_RS19540 Protein ID WP_004142561.1
Coordinates 3970858..3971145 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F28_RS19510 (3966939) 3966939..3967187 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K9F28_RS19515 (3967205) 3967205..3967546 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K9F28_RS19520 (3967577) 3967577..3968692 - 1116 Protein_3842 MBL fold metallo-hydrolase -
K9F28_RS19525 (3968871) 3968871..3969452 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
K9F28_RS19530 (3969452) 3969452..3969820 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
K9F28_RS19535 (3969940) 3969940..3970593 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
K9F28_RS19540 (3970858) 3970858..3971145 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9F28_RS19545 (3971138) 3971138..3971455 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9F28_RS19550 (3971640) 3971640..3972683 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
K9F28_RS19555 (3973350) 3973350..3974216 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
K9F28_RS19560 (3974325) 3974325..3975752 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T215886 WP_004142563.1 NZ_CP083011:c3971455-3971138 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T215886 NZ_CP110996:c1555494-1555159 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT215886 WP_004142561.1 NZ_CP083011:c3971145-3970858 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT215886 NZ_CP110996:c1555742-1555494 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References