Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4002969..4003566 Replicon chromosome
Accession NZ_CP083000
Organism Klebsiella pneumoniae strain 6712.08

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9F30_RS19675 Protein ID WP_004142563.1
Coordinates 4003249..4003566 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9F30_RS19670 Protein ID WP_004142561.1
Coordinates 4002969..4003256 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F30_RS19640 (3999050) 3999050..3999298 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K9F30_RS19645 (3999316) 3999316..3999657 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K9F30_RS19650 (3999688) 3999688..4000803 - 1116 Protein_3867 MBL fold metallo-hydrolase -
K9F30_RS19655 (4000982) 4000982..4001563 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
K9F30_RS19660 (4001563) 4001563..4001931 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
K9F30_RS19665 (4002051) 4002051..4002704 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
K9F30_RS19670 (4002969) 4002969..4003256 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9F30_RS19675 (4003249) 4003249..4003566 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9F30_RS19680 (4003751) 4003751..4004794 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
K9F30_RS19685 (4005461) 4005461..4006327 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
K9F30_RS19690 (4006436) 4006436..4007863 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T215847 WP_004142563.1 NZ_CP083000:c4003566-4003249 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T215847 NZ_CP110978:2643765-2643872 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT215847 WP_004142561.1 NZ_CP083000:c4003256-4002969 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT215847 NZ_CP110978:c2643718-2643652 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References