Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 323979..324565 Replicon chromosome
Accession NZ_CP083000
Organism Klebsiella pneumoniae strain 6712.08

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag K9F30_RS01500 Protein ID WP_002920800.1
Coordinates 324197..324565 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag K9F30_RS01495 Protein ID WP_004174006.1
Coordinates 323979..324200 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F30_RS01475 (320136) 320136..321062 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K9F30_RS01480 (321059) 321059..322336 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
K9F30_RS01485 (322333) 322333..323100 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
K9F30_RS01490 (323102) 323102..323815 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
K9F30_RS01495 (323979) 323979..324200 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K9F30_RS01500 (324197) 324197..324565 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K9F30_RS01505 (324838) 324838..326154 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
K9F30_RS01510 (326261) 326261..327148 + 888 WP_032418302.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
K9F30_RS01515 (327145) 327145..327990 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
K9F30_RS01520 (327992) 327992..329062 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 321059..330143 9084


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T215835 WP_002920800.1 NZ_CP083000:324197-324565 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T215835 NZ_CP110973:327218-327475 [Larkinella insperata]
ATGGTGCAGACGATTCGTAAATTTGGTAATTCGCAGGGTATTCTTCTTCCCCGCACCGTTCTGCAACAGGCTGGTATCGA
GGGAGCCGTCGATATTGAAATAGCCGAAGGCGCGTTAATTCTACGGGCGGTTAAAAGGCACCCCCGAGATGGCTGGGAAG
CCGCCTTCGACGAAGCAATCCAGTCCGGTGACCAACCCGATGGCGACTTGTTTGAGGGTACTTCCAACCAGTTCGACCAA
ACGGAATGGACATGGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT215835 WP_004174006.1 NZ_CP083000:323979-324200 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT215835 NZ_CP110973:327463-327792 [Larkinella insperata]
ATGGACATGGTAATTGGTCGATTTGAGGTCTGGCTGGTTTCACTTGACCCAACCCAGGGCAGCGAAATTGCCAAAACGCG
GCCCTGTCTGGTCGTCTCGCCCAACACAATCAATCAACGGCTCAATACGGTTATGGTGTCCCCAATGACCAGCACGCAGA
AACGGTATCCTACCCGGGTCAATTGCCATTTCGCCGGGCAGGATGGACAGGTGGCGCTGGACCAGACCCGTTCGGTAGAT
AAATCAAGGCTCGTCAAACGGATTGGGAATCTGGATGATGCAACCTGTCACCAAGTGTGTCAAGTGTTGGTGGCCATCTT
CACCTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References