Detailed information of TA system TA021581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4610676..4611280
• Replicon: chromosome
• Accession: NC_008027
• Organism: Pseudomonas entomophila L48

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q1I5S7
• Locus tag: PSEEN_RS19995
• Protein ID: WP_011535379.1
• Coordinates: 4610676..4610996 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PSEEN_RS20000
• Protein ID: WP_011535380.1
• Coordinates: 4610993..4611280 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSEEN_RS19970	4605957..4606178	+	222	WP_011535374.1	cysteine-rich CWC family protein	-
PSEEN_RS19975	4606211..4606960	+	750	WP_011535375.1	16S rRNA pseudouridine(516) synthase	-
PSEEN_RS19980	4607564..4608448	+	885	WP_011535377.1	alpha/beta fold hydrolase	-
PSEEN_RS19990	4608825..4610525	+	1701	WP_011535378.1	SulP family inorganic anion transporter	-
PSEEN_RS19995	4610676..4610996	+	321	WP_011535379.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSEEN_RS20000	4610993..4611280	+	288	WP_011535380.1	helix-turn-helix domain-containing protein	Antitoxin
PSEEN_RS20005	4611319..4611801	-	483	WP_011535381.1	hypothetical protein	-
PSEEN_RS20010	4611816..4612187	-	372	WP_044488403.1	hypothetical protein	-
PSEEN_RS20015	4612284..4613873	-	1590	WP_044488405.1	DUF3327 domain-containing protein	-
PSEEN_RS20020	4613881..4616247	-	2367	WP_011535384.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-87)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12100.03 Da
• Isoelectric Point: 9.9864

>T21581 WP_011535379.1 NC_008027:4610676-4610996 [Pseudomonas entomophila L48]
MLFTETAVFTKRVKELLDDEAYRLLQVRLLIAPDAGDLIEGTGGLRKLRIPANGHGKRGGARVIYYHFNNAAQIAMLYIY
PKIEQADLSTEQRRALKAVIEHWRHP

Nucleotide

• Length: 321 bp

>T21581 NC_008027:4610676-4610996 [Pseudomonas entomophila L48]
ATGCTCTTCACCGAAACCGCCGTCTTCACCAAACGCGTAAAGGAACTGCTCGACGACGAGGCTTACCGGCTGCTGCAGGT
TCGTCTCTTGATTGCACCGGATGCAGGCGACCTCATCGAAGGCACCGGGGGCTTGCGCAAGCTTCGCATTCCAGCCAATG
GGCACGGAAAACGAGGGGGTGCCCGGGTCATCTATTACCACTTCAACAACGCGGCACAGATCGCCATGTTGTACATCTAC
CCCAAGATCGAACAAGCGGACTTGTCCACTGAGCAGCGCAGGGCACTGAAAGCCGTCATCGAACACTGGAGACACCCATG
A

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10976.52 Da
• Isoelectric Point: 7.3221

>AT21581 WP_011535380.1 NC_008027:4610993-4611280 [Pseudomonas entomophila L48]
MNRFFEELMESVEQMDEIIQGQRQPSRQFEIDALQVRNIRKSTGLSQVRFAEVIAVQVATLRNWEQGRREPTGPAKALLR
ALHNDPEHVLKALAH

Nucleotide

• Length: 288 bp

>AT21581 NC_008027:4610993-4611280 [Pseudomonas entomophila L48]
ATGAACCGATTCTTCGAAGAACTGATGGAAAGCGTTGAACAGATGGATGAAATCATCCAGGGCCAACGCCAACCTTCCCG
CCAGTTCGAAATCGATGCGCTTCAAGTCCGCAACATTCGCAAGAGTACCGGCCTGTCCCAGGTACGCTTCGCCGAGGTGA
TCGCTGTGCAGGTCGCCACACTGCGCAACTGGGAGCAAGGCCGCCGCGAACCCACCGGCCCCGCCAAAGCATTGCTTCGA
GCACTGCACAACGACCCAGAGCACGTGCTCAAGGCCCTTGCTCACTGA

Structures

Toxin

• AlphaFold DB: Q1I5S7