Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4023098..4023695 Replicon chromosome
Accession NZ_CP082978
Organism Klebsiella pneumoniae strain BD-13

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9F21_RS19930 Protein ID WP_004142563.1
Coordinates 4023378..4023695 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9F21_RS19925 Protein ID WP_004142561.1
Coordinates 4023098..4023385 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F21_RS19895 (4019179) 4019179..4019427 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K9F21_RS19900 (4019445) 4019445..4019786 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K9F21_RS19905 (4019817) 4019817..4020932 - 1116 Protein_3923 MBL fold metallo-hydrolase -
K9F21_RS19910 (4021111) 4021111..4021692 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
K9F21_RS19915 (4021692) 4021692..4022060 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
K9F21_RS19920 (4022180) 4022180..4022833 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
K9F21_RS19925 (4023098) 4023098..4023385 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9F21_RS19930 (4023378) 4023378..4023695 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9F21_RS19935 (4023880) 4023880..4024923 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
K9F21_RS19940 (4025590) 4025590..4026456 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
K9F21_RS19945 (4026565) 4026565..4027992 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T215768 WP_004142563.1 NZ_CP082978:c4023695-4023378 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T215768 NZ_CP110942:53921-54403 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT215768 WP_004142561.1 NZ_CP082978:c4023385-4023098 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT215768 NZ_CP110942:53667-53933 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References