Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4003783..4004380 Replicon chromosome
Accession NZ_CP082967
Organism Klebsiella pneumoniae strain BD-41

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9F23_RS19760 Protein ID WP_004142563.1
Coordinates 4004063..4004380 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9F23_RS19755 Protein ID WP_004142561.1
Coordinates 4003783..4004070 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F23_RS19725 (3999864) 3999864..4000112 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K9F23_RS19730 (4000130) 4000130..4000471 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K9F23_RS19735 (4000502) 4000502..4001617 - 1116 Protein_3883 MBL fold metallo-hydrolase -
K9F23_RS19740 (4001796) 4001796..4002377 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
K9F23_RS19745 (4002377) 4002377..4002745 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
K9F23_RS19750 (4002865) 4002865..4003518 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
K9F23_RS19755 (4003783) 4003783..4004070 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9F23_RS19760 (4004063) 4004063..4004380 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9F23_RS19765 (4004565) 4004565..4005608 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
K9F23_RS19770 (4006275) 4006275..4007141 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
K9F23_RS19775 (4007250) 4007250..4008677 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T215729 WP_004142563.1 NZ_CP082967:c4004380-4004063 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T215729 NZ_CP110933:c47644-47339 [Salmonella enterica]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGAACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT215729 WP_004142561.1 NZ_CP082967:c4004070-4003783 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT215729 NZ_CP110933:c47864-47646 [Salmonella enterica]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References