Detailed information of TA system TA021569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1408222..1408863
• Replicon: plasmid megaplasmid
• Accession: NC_007974
• Organism: Cupriavidus metallidurans CH34

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q1LE01
• Locus tag: RMET_RS24475
• Protein ID: WP_011519196.1
• Coordinates: 1408222..1408506 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RMET_RS24480
• Protein ID: WP_011519197.1
• Coordinates: 1408516..1408863 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RMET_RS24445	1403238..1403639	+	402	WP_011519190.1	hypothetical protein	-
RMET_RS24450	1403722..1404312	-	591	WP_011519191.1	hypothetical protein	-
RMET_RS24455	1404435..1405121	-	687	WP_011519192.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
RMET_RS24460	1405474..1406364	-	891	WP_011519194.1	helix-turn-helix transcriptional regulator	-
RMET_RS24465	1406674..1406892	+	219	WP_008651241.1	YdcH family protein	-
RMET_RS24470	1406894..1408045	+	1152	WP_011519195.1	polyphosphate kinase 2	-
RMET_RS24475	1408222..1408506	+	285	WP_011519196.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RMET_RS24480	1408516..1408863	+	348	WP_011519197.1	HigA family addiction module antidote protein	Antitoxin
RMET_RS24485	1408978..1410291	+	1314	WP_011519198.1	hemolysin family protein	-
RMET_RS24490	1410691..1412028	+	1338	WP_011519199.1	EAL domain-containing protein	-
RMET_RS24495	1412173..1412301	+	129	WP_008651235.1	entericidin A/B family lipoprotein	-
RMET_RS24500	1412420..1413061	-	642	WP_011519200.1	bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	motA / cheW / cheB / cheY / flhA / flgC / flgG / acrB / plc / adeG / fliI / fliP / katA / katA / kdsA	1..2580084	2580084

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (23-99)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10505.15 Da
• Isoelectric Point: 8.5384

>T21569 WP_011519196.1 NC_007974:1408222-1408506 [Cupriavidus metallidurans CH34]
MIRSLSDKTTAAIYCGQSVHTLPAHIQADALRKLAILDAASSLPDMVAPPANRLEPVLGKRRGHWRVRIKGPWHLCFRFI
NGEAWDVGIAEYEE

Nucleotide

• Length: 285 bp

>T21569 NC_007974:1408222-1408506 [Cupriavidus metallidurans CH34]
ATGATCCGCTCGCTCTCCGACAAGACAACGGCCGCCATCTATTGCGGCCAGTCCGTTCACACACTGCCCGCTCACATTCA
GGCCGACGCGCTGCGCAAGCTCGCGATCCTCGACGCTGCGTCGTCTCTTCCCGATATGGTCGCCCCACCCGCAAACCGGC
TGGAACCAGTTCTTGGCAAGCGCCGCGGACACTGGCGCGTCCGCATTAAAGGTCCGTGGCATCTCTGTTTTCGCTTCATC
AACGGCGAAGCCTGGGACGTCGGCATCGCCGAATACGAGGAATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12691.46 Da
• Isoelectric Point: 7.3198

>AT21569 WP_011519197.1 NC_007974:1408516-1408863 [Cupriavidus metallidurans CH34]
MTINREDLRRADFRSIATGQPIPPAHPGDVLETEFMAPNELSAEALAVALHMAPQRIHDIVRRQRAISPDTALRLARYFG
TSAEFWAGLQTAYALRLARATAGANIERDIQPLTA

Nucleotide

• Length: 348 bp

>AT21569 NC_007974:1408516-1408863 [Cupriavidus metallidurans CH34]
ATGACAATCAACCGCGAAGACTTGCGGCGCGCGGATTTTCGCAGCATTGCCACCGGCCAGCCGATTCCGCCGGCACACCC
CGGCGATGTACTGGAAACCGAGTTCATGGCGCCAAACGAACTGTCGGCGGAAGCATTGGCGGTGGCCTTGCATATGGCGC
CGCAACGAATTCACGATATCGTGCGGCGTCAGCGAGCCATCTCGCCAGACACCGCCCTGCGGCTGGCCCGCTATTTCGGC
ACTAGTGCGGAATTCTGGGCGGGCCTCCAGACCGCATATGCACTGCGGCTGGCGCGCGCGACTGCAGGGGCGAACATCGA
GCGCGACATCCAGCCGCTGACAGCATGA

Structures

Toxin

• AlphaFold DB: A0A132H834