Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4317979..4318565 Replicon chromosome
Accession NZ_CP082936
Organism Klebsiella pneumoniae strain NDM-71

Toxin (Protein)


Gene name doc Uniprot ID A0A486RB10
Locus tag K8M01_RS20860 Protein ID WP_004174007.1
Coordinates 4318197..4318565 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag K8M01_RS20855 Protein ID WP_004174006.1
Coordinates 4317979..4318200 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M01_RS20835 (4314136) 4314136..4315062 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K8M01_RS20840 (4315059) 4315059..4316336 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
K8M01_RS20845 (4316333) 4316333..4317100 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
K8M01_RS20850 (4317102) 4317102..4317815 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
K8M01_RS20855 (4317979) 4317979..4318200 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K8M01_RS20860 (4318197) 4318197..4318565 + 369 WP_004174007.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K8M01_RS20865 (4318838) 4318838..4320154 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
K8M01_RS20870 (4320261) 4320261..4321148 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
K8M01_RS20875 (4321145) 4321145..4321990 + 846 WP_004174009.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
K8M01_RS20880 (4321992) 4321992..4323062 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4301886..4323799 21913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13568.97 Da        Isoelectric Point: 8.6410

>T215669 WP_004174007.1 NZ_CP082936:4318197-4318565 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDLGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T215669 NZ_CP110886:c4453588-4453508 [Dickeya solani]
AATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGGGCACAGGAATCGTGTTCGGTCTTTTTT
T

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT215669 WP_004174006.1 NZ_CP082936:4317979-4318200 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT215669 NZ_CP110886:4453506-4453588 [Dickeya solani]
ATAAAAAAAGACCGAACACGATTCCTGTGCCCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486RB10

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References