Detailed information of TA system TA215650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4474248..4474850
• Replicon: chromosome
• Accession: NZ_CP082929
• Organism: Salmonella enterica subsp. enterica serovar London isolate N18-04674

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M7S4R6
• Locus tag: K6991_RS21535
• Protein ID: WP_001159635.1
• Coordinates: 4474539..4474850 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6991_RS21530
• Protein ID: WP_000362050.1
• Coordinates: 4474248..4474538 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6991_RS21515 (4471741)	4471741..4472643	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
K6991_RS21520 (4472640)	4472640..4473275	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
K6991_RS21525 (4473272)	4473272..4474201	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
K6991_RS21530 (4474248)	4474248..4474538	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
K6991_RS21535 (4474539)	4474539..4474850	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
K6991_RS21540 (4475068)	4475068..4475997	+	930	WP_023255277.1	alpha/beta hydrolase	-
K6991_RS21545 (4476083)	4476083..4476394	+	312	WP_000558163.1	type II toxin-antitoxin system HigB family toxin	-
K6991_RS21550 (4476391)	4476391..4476837	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
K6991_RS21555 (4476852)	4476852..4477793	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
K6991_RS21560 (4477838)	4477838..4478275	-	438	WP_001621365.1	D-aminoacyl-tRNA deacylase	-
K6991_RS21565 (4478272)	4478272..4479144	-	873	WP_023255278.1	virulence factor BrkB family protein	-
K6991_RS21570 (4479138)	4479138..4479737	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12340.30 Da
• Isoelectric Point: 9.4460

>T215650 WP_001159635.1 NZ_CP082929:c4474850-4474539 [Salmonella enterica subsp. enterica serovar London]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T215650 NZ_CP110873:588387-588728 [Enterobacter kobei]
ATGAAAACTCTACCTGCAACAACGCCGCAGGCGGTGACGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTTCGTATTGATCGTAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGGCAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCTCAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT215650 WP_000362050.1 NZ_CP082929:c4474538-4474248 [Salmonella enterica subsp. enterica serovar London]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT215650 NZ_CP110873:588025-588366 [Enterobacter kobei]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCCAGTGGGGGCTGAATCGTAATGTGACGCCTTGCTTTGGCGCTCG
TTTGGTACAGGAGGGAAACCGGCTGCACTATCTGATTGACCGGGCCAGCATTACCGGTGACTTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CATTTACCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: A0A3V2JK97