Detailed information of TA system TA021562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4393330..4393934
• Replicon: chromosome
• Accession: NC_007964
• Organism: Nitrobacter hamburgensis X14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q1QGF1
• Locus tag: NHAM_RS20640
• Protein ID: WP_011512323.1
• Coordinates: 4393650..4393934 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NHAM_RS20635
• Protein ID: WP_011512322.1
• Coordinates: 4393330..4393635 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAM_RS20605	4388588..4389358	-	771	WP_011512319.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
NHAM_RS27100	4389801..4390187	+	387	WP_157043717.1	hypothetical protein	-
NHAM_RS20615	4390452..4391627	-	1176	WP_011512321.1	DUF3644 domain-containing protein	-
NHAM_RS20625	4391855..4392979	-	1125	WP_041358512.1	hypothetical protein	-
NHAM_RS26215	4393014..4393235	-	222	WP_157043718.1	hypothetical protein	-
NHAM_RS20635	4393330..4393635	-	306	WP_011512322.1	HigA family addiction module antidote protein	Antitoxin
NHAM_RS20640	4393650..4393934	-	285	WP_011512323.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NHAM_RS20645	4394170..4395075	-	906	WP_011512324.1	DUF1738 domain-containing protein	-
NHAM_RS20650	4395727..4395996	+	270	WP_011512325.1	hypothetical protein	-
NHAM_RS25460	4396150..4396347	+	198	Protein_4103	ParA family protein	-
NHAM_RS27105	4397217..4397615	+	399	WP_157043720.1	hypothetical protein	-
NHAM_RS25475	4397643..4398023	+	381	WP_081435050.1	hypothetical protein	-
NHAM_RS20660	4398068..4398622	-	555	WP_157043721.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (29-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-66)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10895.36 Da
• Isoelectric Point: 8.5273

>T21562 WP_011512323.1 NC_007964:c4393934-4393650 [Nitrobacter hamburgensis X14]
MILNYRDKRTQAFAAGTLVREFQGFDRQAWKRLEILDAATSLDDLRGLPSNRLEALRGDRRGQFCIRINAQWRICFEWPQ
GAAGPSQVEIVDYH

Nucleotide

• Length: 285 bp

>T21562 NC_007964:c4393934-4393650 [Nitrobacter hamburgensis X14]
ATGATTCTGAACTACCGCGACAAACGGACCCAAGCCTTCGCTGCCGGCACGTTGGTCCGGGAGTTTCAGGGGTTCGACCG
GCAGGCGTGGAAGCGCCTTGAAATCCTCGATGCCGCGACCAGCCTGGACGATCTGCGGGGCTTGCCCAGCAACCGTCTTG
AGGCGTTGCGCGGCGACAGGCGCGGCCAGTTCTGCATCCGCATCAATGCACAATGGCGGATTTGCTTTGAATGGCCGCAA
GGCGCTGCCGGGCCGTCGCAGGTCGAAATCGTGGACTATCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11171.90 Da
• Isoelectric Point: 10.9867

>AT21562 WP_011512322.1 NC_007964:c4393635-4393330 [Nitrobacter hamburgensis X14]
MARPPIHPGEILADELQEIGITPTELARQIKVPANRITQIIHGRRNITGDTALRLGHWFGASAQFWLNLQSAYDIRMAQA
KAGREIAKLPVRREPASVGTP

Nucleotide

• Length: 306 bp

>AT21562 NC_007964:c4393635-4393330 [Nitrobacter hamburgensis X14]
ATGGCACGGCCCCCCATACACCCCGGCGAAATCCTCGCCGACGAATTGCAGGAAATCGGCATCACGCCGACCGAGCTGGC
GCGCCAGATCAAGGTGCCCGCCAACCGCATCACGCAGATCATTCACGGCCGGCGCAACATCACCGGCGACACCGCCCTGC
GGCTCGGCCACTGGTTCGGCGCGTCGGCGCAGTTCTGGCTCAATCTCCAGAGCGCTTACGATATTCGCATGGCCCAGGCG
AAGGCCGGCCGCGAAATCGCCAAGCTCCCCGTCCGGCGCGAGCCGGCCAGCGTCGGCACCCCATAA

Structures

Toxin

• AlphaFold DB: Q1QGF1