Detailed information of TA system TA021561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3145309..3145915
• Replicon: chromosome
• Accession: NC_007964
• Organism: Nitrobacter hamburgensis X14

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q1QJH8
• Locus tag: NHAM_RS14610
• Protein ID: WP_011511283.1
• Coordinates: 3145309..3145590 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: NHAM_RS14615
• Protein ID: WP_011511284.1
• Coordinates: 3145613..3145915 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAM_RS14585	3140494..3141769	-	1276	Protein_2902	IS256 family transposase	-
NHAM_RS14590	3141867..3142139	-	273	Protein_2903	Rha family transcriptional regulator	-
NHAM_RS14595	3142276..3142698	-	423	WP_011511281.1	twin-arginine translocation signal domain-containing protein	-
NHAM_RS26800	3143807..3143950	-	144	WP_157043642.1	hypothetical protein	-
NHAM_RS14600	3144236..3144556	-	321	WP_041358190.1	hypothetical protein	-
NHAM_RS14605	3144786..3145079	-	294	WP_041358191.1	hypothetical protein	-
NHAM_RS14610	3145309..3145590	+	282	WP_011511283.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NHAM_RS14615	3145613..3145915	+	303	WP_011511284.1	HigA family addiction module antidote protein	Antitoxin
NHAM_RS14620	3146157..3146453	+	297	WP_041358192.1	hypothetical protein	-
NHAM_RS14625	3146474..3146806	-	333	WP_011511286.1	hypothetical protein	-
NHAM_RS14635	3147035..3147421	+	387	WP_011511287.1	ribonuclease HI	-
NHAM_RS14640	3147418..3147666	+	249	WP_011511288.1	hypothetical protein	-
NHAM_RS25040	3147663..3147872	+	210	Protein_2914	AlpA family phage regulatory protein	-
NHAM_RS14645	3147919..3148998	+	1080	WP_011511289.1	tyrosine-type recombinase/integrase	-
NHAM_RS14655	3149148..3149480	-	333	WP_011511290.1	hypothetical protein	-
NHAM_RS14660	3149479..3150552	+	1074	WP_041358196.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3140494..3141633	1139

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10533.04 Da
• Isoelectric Point: 9.6547

>T21561 WP_011511283.1 NC_007964:3145309-3145590 [Nitrobacter hamburgensis X14]
MIRSFKDSLAEDAFNGVVRKGFPANLIKIARRKLNYLDAAKALDDLKAPPGNRLEALTGGRKGQHSIRVNDQFRICFVWT
DEGPKDVEFVDYH

Nucleotide

• Length: 282 bp

>T21561 NC_007964:3145309-3145590 [Nitrobacter hamburgensis X14]
ATGATCAGGAGCTTCAAAGACAGCCTGGCCGAAGACGCCTTTAATGGCGTGGTTCGCAAAGGCTTCCCGGCCAACTTGAT
CAAGATTGCTCGACGCAAACTTAACTATCTCGATGCGGCCAAGGCTCTCGATGACTTGAAAGCGCCGCCCGGCAATCGTC
TTGAGGCTTTGACCGGAGGCCGCAAGGGTCAGCATTCGATCCGCGTTAACGATCAGTTCCGCATCTGCTTTGTTTGGACC
GATGAAGGACCGAAGGACGTAGAGTTTGTCGACTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11192.07 Da
• Isoelectric Point: 9.0798

>AT21561 WP_011511284.1 NC_007964:3145613-3145915 [Nitrobacter hamburgensis X14]
MAKKLPPMHPGEVLREEFLVPMHLSAGKLAKACGIPRTRIERIANEQIGITADTALRLAKAFETTTELWLNLQNSYDIRV
AKEQIGKDLDKIEPITQRAA

Nucleotide

• Length: 303 bp

>AT21561 NC_007964:3145613-3145915 [Nitrobacter hamburgensis X14]
ATGGCTAAAAAGCTTCCACCCATGCACCCTGGGGAAGTCCTCCGAGAGGAGTTCCTTGTCCCGATGCATCTTTCGGCCGG
CAAGCTCGCGAAAGCTTGCGGCATTCCGCGCACTCGGATCGAGCGGATCGCCAATGAACAGATTGGCATCACTGCGGATA
CAGCCCTGCGTCTCGCCAAAGCCTTCGAGACGACAACGGAGCTTTGGCTAAACCTTCAAAACTCCTACGATATTCGAGTC
GCCAAGGAACAGATCGGCAAAGACCTCGATAAAATCGAGCCGATTACACAGCGCGCCGCGTAA

Structures

Toxin

• AlphaFold DB: Q1QJH8