Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2918333..2919060 Replicon chromosome
Accession NZ_CP082904
Organism Mixta hanseatica isolate X22927

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag K6958_RS13865 Protein ID WP_249891684.1
Coordinates 2918749..2919060 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K6958_RS13860 Protein ID WP_249891683.1
Coordinates 2918333..2918752 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6958_RS13840 (K6958_13840) 2913956..2915086 + 1131 WP_085071046.1 class Ia ribonucleoside-diphosphate reductase subunit beta -
K6958_RS13845 (K6958_13845) 2915089..2915349 + 261 WP_249894696.1 class I ribonucleotide reductase maintenance protein YfaE -
K6958_RS13850 (K6958_13850) 2915406..2916602 - 1197 WP_249891677.1 nicotinamide mononucleotide deamidase-related protein YfaY -
K6958_RS13855 (K6958_13855) 2916820..2918262 + 1443 WP_249891679.1 catalase -
K6958_RS13860 (K6958_13860) 2918333..2918752 - 420 WP_249891683.1 helix-turn-helix domain-containing protein Antitoxin
K6958_RS13865 (K6958_13865) 2918749..2919060 - 312 WP_249891684.1 type II toxin-antitoxin system HigB family toxin Toxin
K6958_RS13870 (K6958_13870) 2919175..2920581 - 1407 WP_249891685.1 o-succinylbenzoate--CoA ligase -
K6958_RS13875 (K6958_13875) 2920563..2921525 - 963 WP_249891686.1 o-succinylbenzoate synthase -
K6958_RS13880 (K6958_13880) 2921525..2922382 - 858 WP_249891687.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
K6958_RS13885 (K6958_13885) 2922398..2923168 - 771 WP_249891688.1 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12377.17 Da        Isoelectric Point: 9.9441

>T215526 WP_249891684.1 NZ_CP082904:c2919060-2918749 [Mixta hanseatica]
MHVISKEPFEQAARLFPNDATAIKALYRLVRETDFKSPDELRAAVPSLDNFKYRNKWWVLDVGGNHLRVIVYINFVNKRF
YVKHIATHTEYDKLTRYYRENKE

Download         Length: 312 bp

>T215526 NZ_CP110809:c2089937-2089653 [Pseudomonas syringae]
ATGCAGGTTGAGTGGCTCAAAACAGCACTAAAGAATCTGGATGAGGAGGCAGCGTATATCGCACTGGACAATCCAGCTGC
TGCCGCTGCTTTCGTGAAAGCCATTCAATCAAGCGTAACGCAGCTCGCGAGCTTTCCCGCCATGGGCCGGGAAGGACGGA
TTGCCGGAACCAGAGAATGGCCTCTGCCAGACCTGCCTTACCTGATTCCATATCGCATCCGTAGCGGCCGACTACAGGTT
CTACGGATTTTCCACGCCAGAAGACAATCGCCACCCGTGTGGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15396.64 Da        Isoelectric Point: 5.1787

>AT215526 WP_249891683.1 NZ_CP082904:c2918752-2918333 [Mixta hanseatica]
MIADTTKAIEATKQLVAAVPFLGSSVSEKDYRDALALVNYLIDNDDENPLIDFLTVKIAAYEDNSEHFAAFNKAVEEMPV
GVALLRTLIDQHKLSYSDLKQEIGSKSLVSQILSGQRSLTISHIKALSVRFGVKPQWFL

Download         Length: 420 bp

>AT215526 NZ_CP110809:c2090166-2089927 [Pseudomonas syringae]
ATGTCCACAATGTCTCTACGTCTGCCGGACGAAATGGCAGATACCCTTGCACACCTTGCAAAGGCGACCGGGCGCAGCAA
GTCATTTTTGGCCCTCGACGCGCTCCGCGAATACCTGACACGCGAGGCTTGGCAGATTGCCGAAATTCAGCGTGCCATCG
AAGAAGCCGACGCCGGCGAGTTCGCAAGCCAAGAAGACGTAAAAGCTGTCATGGACAAATGGTCAGGCAATGCAGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References