Detailed information of TA system TA215499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2889..3540
• Replicon: plasmid p2014LNAB1-3
• Accession: NZ_CP082890
• Organism: Acinetobacter baumannii strain 2014LNAB1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: H1I72_RS20680
• Protein ID: WP_000269904.1
• Coordinates: 2889..3245 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H1I72_RS20685
• Protein ID: WP_001129974.1
• Coordinates: 3238..3540 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1I72_RS20665	283..1218	+	936	WP_001292329.1	replication initiation protein RepM	-
H1I72_RS20670	1296..1814	+	519	WP_000839337.1	plasmid replication DNA-binding protein	-
H1I72_RS20675	2024..2323	+	300	WP_000358348.1	hypothetical protein	-
H1I72_RS20680	2889..3245	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H1I72_RS20685	3238..3540	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
H1I72_RS20690	3533..3742	+	210	WP_000069474.1	hypothetical protein	-
H1I72_RS20695	4068..4529	-	462	WP_000761346.1	DIP1984 family protein	-
H1I72_RS20700	4834..7245	-	2412	WP_225370903.1	TonB-dependent receptor ZnuD2	-
H1I72_RS20705	7373..7687	-	315	WP_001983304.1	BrnA antitoxin family protein	-
H1I72_RS20710	7674..7961	-	288	WP_000438827.1	BrnT family toxin	-
H1I72_RS20715	8229..8528	-	300	WP_000358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11194	11194

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T215499 WP_000269904.1 NZ_CP082890:2889-3245 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T215499 NZ_CP110790:c4226769-4225474 [Yersinia massiliensis]
ATGGAAAAGTTAACCCTTGCATTAAACGGCATCGTAGTTGGTACCTTGGAGAAGGCTGCCGGTGGTGAAATCTCTTTTTT
CTATCATGATAGCTGGCTTGAACGCAGTGGAGCACGTGCGATTTCTCTGTCGTTACCCCTACAAACGGTGAAATTCCGTG
GGGAGGTTGTTTATAATTTTTTTGATAACCTTCTGCCTGACAGTGAATCTATTCGCAGCCGTATTCAAGCGCGTTTTCAA
ACACCAACAAAGCAACCTTTTGATTTACTCTCCCAAATAGGGAGTGATTGCGTAGGGGCAATTCAATTATACCCAGAAGG
CCGGCAAATAAATTCTGTCACCGAGATCCATGCAACGCCTTTAAATGACAATGAGATAGAAAAATTACTCTTAGGGTATA
AGGAGGCGCCTTTGGGCATGGAGGCCGATATTGATGATTTCCGTATTTCTTTGGCTGGGGCACAAGAGAAAACAGCCTTA
CTGTGGTATCAGAATCAATGGCATCGGCCAGAAGGGAGTACACCAACGAGTCATATTCTTAAATTGCCTATAGGCTATAT
AGCCGCTAATGGTATTGATTTAAGTGAAAGTGTCGAGAATGAATGGTTGTGCTTAAAGATTGCTGAAGAATTTGGTCTAC
CTGTCGCGTATTCAGAAATAAAATATTTTGGTAAGCAAAAGGCATTAGTTGTTGAGCGGTTCGATCGCCGCTGGAGCCCT
AATAATAGTTGGTTGATGCGTTTACCGCAGGAGGATTTTTGTCAGGTCTTAGGTGTAGCACCTGCACTCAAATATGAATC
TGATGGTGGTCCTGGGATTGCAGATGCGATGAAACTATTACTCGGTTCTCAGCAAGCGATTCAGGATCGGGATATTTTTT
TTAAAAGTCAGATACTTTTCTGGATGTTAGCCGCGATTGACGGGCACGCGAAGAATTTTAGTGTGTTTATTGAATTGGAT
AGCGCATTTCGTATGACACCCATTTACGATGTGATGTCTGCATTTCCACTCATGCACCCACGAGGAATACGGGCTAAAAA
AGCAAAAATGGCCATGGCATTACTTGGACGTAATCGGCATTTTCACTGGAGTACTATTTTACCTCGGCACTTCATTTCAA
CCGCGAAACAGGTGAATTATCCCGAAGATAATGTTGTGCAATTAATGGCTGAAATGAAAGAAAAAACCGAACAAGTGATA
GCTGCGGTGAAGGCGGTTCTACCACAGGGCTTTCCCGAAAAAATCAGTGAGCCTATTTTCTCTGGTTTAAGGCGGCAGTC
GGCGAAACTGCCCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT215499 WP_001129974.1 NZ_CP082890:3238-3540 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT215499 NZ_CP110790:c4227024-4226773 [Yersinia massiliensis]
ATGAAAATTACCTCTGCTGCAATGTTGGCAAATACACTACGGAATGAACGCAAAAAAAGTAAGCTGACTCAGCGAAAAAC
AGCCGAGACAGTCGGTTTGAAGCAATCAACAGTATCAGAGTTTGAAAACCATCCTGATGGTACCAAATTAGACACGCTGT
TTAAGTTATTGGCAGCACTGGATTTAGAGCTACAGGTTAGGCCAAGGAACCGCAATATTAAGGATAATACAACCTGGGAT
CAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1