Detailed information of TA system TA215469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1461513..1462152
• Replicon: chromosome
• Accession: NZ_CP082857
• Organism: Haemophilus aegyptius strain FDAARGOS_1478

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K8O83_RS07800
• Protein ID: WP_005650215.1
• Coordinates: 1461847..1462152 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8O83_RS07795
• Protein ID: WP_005650217.1
• Coordinates: 1461513..1461836 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8O83_RS07775 (K8O83_07680)	1457133..1457306	-	174	WP_005657572.1	DUF5363 domain-containing protein	-
K8O83_RS07780 (K8O83_07685)	1457303..1459273	-	1971	WP_006995880.1	GNAT family N-acetyltransferase	-
K8O83_RS07785 (K8O83_07690)	1459278..1459619	-	342	WP_006995879.1	SirB2 family protein	-
K8O83_RS07790 (K8O83_07695)	1459680..1461350	-	1671	WP_005663485.1	energy-dependent translational throttle protein EttA	-
K8O83_RS07795 (K8O83_07700)	1461513..1461836	-	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K8O83_RS07800 (K8O83_07705)	1461847..1462152	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8O83_RS07805 (K8O83_07710)	1462477..1463097	+	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
K8O83_RS07810 (K8O83_07715)	1463100..1464074	+	975	WP_006995878.1	zinc transporter permease subunit ZevB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T215469 WP_005650215.1 NZ_CP082857:c1462152-1461847 [Haemophilus aegyptius]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T215469 NZ_CP110777:c110576-110046 [Citrobacter freundii]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT215469 WP_005650217.1 NZ_CP082857:c1461836-1461513 [Haemophilus aegyptius]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT215469 NZ_CP110777:c110849-110580 [Citrobacter freundii]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70