Detailed information of TA system TA215463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1561622..1562261
• Replicon: chromosome
• Accession: NZ_CP082856
• Organism: Haemophilus influenzae strain FDAARGOS_1479

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: K8P04_RS07735
• Protein ID: WP_005650215.1
• Coordinates: 1561622..1561927 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8P04_RS07740
• Protein ID: WP_005650217.1
• Coordinates: 1561938..1562261 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8P04_RS07715 (K8P04_07640)	1557052..1559091	-	2040	WP_005646675.1	excinuclease ABC subunit UvrB	-
K8P04_RS07725 (K8P04_07650)	1559706..1560674	-	969	WP_015702144.1	zinc transporter permease subunit ZevB	-
K8P04_RS07730 (K8P04_07655)	1560677..1561297	-	621	WP_005650213.1	zinc transporter binding subunit ZevA	-
K8P04_RS07735 (K8P04_07660)	1561622..1561927	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8P04_RS07740 (K8P04_07665)	1561938..1562261	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
K8P04_RS07745 (K8P04_07670)	1562424..1564094	+	1671	WP_015702145.1	energy-dependent translational throttle protein EttA	-
K8P04_RS07750 (K8P04_07675)	1564156..1564497	+	342	WP_005666735.1	SirB2 family protein	-
K8P04_RS07755 (K8P04_07680)	1564502..1566346	+	1845	WP_015702146.1	GNAT family N-acetyltransferase	-
K8P04_RS07760 (K8P04_07685)	1566343..1566516	+	174	WP_005652999.1	DUF5363 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T215463 WP_005650215.1 NZ_CP082856:1561622-1561927 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T215463 NZ_CP110775:3461652-3462026 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT215463 WP_005650217.1 NZ_CP082856:1561938-1562261 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT215463 NZ_CP110775:3461410-3461652 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGACAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A806EC70