Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 8524..9263 Replicon plasmid unnamed2
Accession NZ_CP082854
Organism Legionella anisa strain FDAARGOS_1480

Toxin (Protein)


Gene name higB Uniprot ID A0A2Z3KTI3
Locus tag K8O89_RS19130 Protein ID WP_019232997.1
Coordinates 8524..8835 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2Z3L5D3
Locus tag K8O89_RS19135 Protein ID WP_019232998.1
Coordinates 8832..9263 (+) Length 144 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8O89_RS19105 (K8O89_19025) 4101..4337 - 237 WP_080480132.1 hypothetical protein -
K8O89_RS19110 (K8O89_19030) 4349..4606 - 258 WP_127908205.1 hypothetical protein -
K8O89_RS19115 (K8O89_19035) 4622..4918 - 297 WP_019232994.1 hypothetical protein -
K8O89_RS19120 (K8O89_19040) 5144..7813 + 2670 WP_019232995.1 Ti-type conjugative transfer relaxase TraA -
K8O89_RS19125 (K8O89_19045) 7824..8435 + 612 WP_019232996.1 hypothetical protein -
K8O89_RS19130 (K8O89_19050) 8524..8835 + 312 WP_019232997.1 type II toxin-antitoxin system HigB family toxin Toxin
K8O89_RS19135 (K8O89_19055) 8832..9263 + 432 WP_019232998.1 transcriptional regulator Antitoxin
K8O89_RS19140 (K8O89_19060) 9300..11165 - 1866 WP_019232999.1 type IV conjugative transfer system coupling protein TraD -
K8O89_RS19145 (K8O89_19065) 11165..11920 - 756 WP_019233000.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..48353 48353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Antitoxin HigA

(1-143)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.55 Da        Isoelectric Point: 10.2563

>T215455 WP_019232997.1 NZ_CP082854:8524-8835 [Legionella anisa]
MHVISKKPFVEAAKKYPNQRQAILDLYRVLDKITVNTPLEMKAIFPSLDNFKYKDKWWIIDIGGNHLRLMAFIQFSQNRI
YVKHIVTHAEYDRLCEQYRRNKQ

Download         Length: 312 bp

>T215455 NZ_CP110774:c1787990-1787472 [Acidocella sp. MX-AZ03]
GTGAGACGTCGGTTGTTGCCGCCGGAACGCCTGACCGCAGCACACGATGTCTCAGGCTTCCAGAATGGCCGCCACCCGTC
CTTGGACGAATGGCTGCGGGACCGGGCATTGGTCAGTGAGGGGCTATCAGCGAGAACCTATGTTGTACGCGCTGGCGAGC
CGCCACATCTCGTCGTCGGCTATTATGCGATCACCACGGCCATGGAGCAGCGCTTGTCCCTGCCCAGCGCCAAGCTGCGG
CGCGGAATGCCCGAGCAGATACCGCTGATGCTAATCGGCCGCTTGGCGGTGGACCAGCGTTACCATGGCATTGGCCTGGG
CACCGACTTATTGGCGGATGCATTGAAGCGTTGCCTCGCCGTGTCCGAGGTGGCGGGCGTCAGGGCGGTGGTCACCCACG
CGATCGATGAGACCGCCATGGCGTTCTATCGGCGGCACGGCTTTTTGGCCTCGCCGTTGGGCGAGAAGGTGCTGATGATG
CCGATTGAGACCATCCGCGCCGTGTTCGCCGATCAGTGA

Antitoxin


Download         Length: 144 a.a.        Molecular weight: 16294.65 Da        Isoelectric Point: 4.7426

>AT215455 WP_019232998.1 NZ_CP082854:8832-9263 [Legionella anisa]
MISEQVILNKFIDFTKVAAPVLSLKDEQAYEDALTMMEHLMESVGEQRNKPENILIGLLAHAIEEYESKDEEISDFVSQS
MSDRGDVALLRMIIDQHQLTLSDLPEVGHKSLISKILSGERNLTRSHIEKLSKRFHIDPGLFF

Download         Length: 432 bp

>AT215455 NZ_CP110774:c1788325-1788032 [Acidocella sp. MX-AZ03]
ATGGCCCAGCCAGCCCCTGCCGGTCCGGCCCGTAAGGACGATGTCATTCAGATCCGAGCCTCGGCTGAGACCAAGGCAAT
CCTGACCCGCGCCGCGGCGCTGCGGGGGCAGAAGCTGTCGGAATTCATGCTGGACAGCGCCCGCCGCCAGGCCGAGGACA
CGATCCTGGATCAGCGTGTGTTCTTCCTGGCGCCCGATGCGCATGAGGCGTTTCTGGCGATGCTGGATGCGCCGGCACAG
GACGTGGCGGAACTGCGGGCGCGGCTGACCAAGCCGCCAGTCTGGGACCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3KTI3

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3L5D3

References