Detailed information of TA system TA215445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 791569..792190
• Replicon: chromosome
• Accession: NZ_CP082847
• Organism: Legionella pneumophila strain FDAARGOS_1483

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q5ZRG7
• Locus tag: K8P08_RS03670
• Protein ID: WP_010948600.1
• Coordinates: 791569..791790 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8P08_RS03675
• Protein ID: WP_010948599.1
• Coordinates: 791900..792190 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8P08_RS03650 (K8P08_03710)	786731..787909	-	1179	WP_010948604.1	serine hydrolase domain-containing protein	-
K8P08_RS03655 (K8P08_03715)	788439..789401	+	963	WP_015443886.1	lytic murein transglycosylase	-
K8P08_RS03660 (K8P08_03720)	789705..790751	+	1047	WP_010948602.1	FAD-dependent oxidoreductase	-
K8P08_RS03665 (K8P08_03725)	790861..791364	+	504	WP_015443887.1	hypothetical protein	-
K8P08_RS03670 (K8P08_03730)	791569..791790	+	222	WP_010948600.1	hypothetical protein	Toxin
K8P08_RS03675 (K8P08_03735)	791900..792190	+	291	WP_010948599.1	helix-turn-helix domain-containing protein	Antitoxin
K8P08_RS03680 (K8P08_03740)	792479..792670	-	192	WP_014327021.1	hypothetical protein	-
K8P08_RS03685 (K8P08_03745)	793038..794480	+	1443	WP_010948598.1	ATP-binding protein	-
K8P08_RS03690 (K8P08_03750)	794642..796129	-	1488	WP_010948597.1	lpg2912 family Dot/Icm T4SS effector	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-88)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8288.46 Da
• Isoelectric Point: 3.7468

>T215445 WP_010948600.1 NZ_CP082847:791569-791790 [Legionella pneumophila]
MIFIETPIFTEDVKELLSDEEYAEFQEYLADNPLAGDVIQQTGKLANWQTAKSSMVISRQMGSEAVLELFIIM

Nucleotide

• Length: 222 bp

>T215445 NZ_CP110678:2168497-2168769 [Sphingomonas sp. S1-29]
ATGGCGTACATTGTCGACATGGAAAGCCTGAGCATCTCCGAAGCGCGCGCCAATATGAAGGCGGTGGTCGATCGCGTCGT
GGCCGACAAGGCACCGGTGGCGATCACCCGCCAGCGCGGCGAGGGCGTGGTGATGATCTCGGCGAGCGAATGGGCGTCGA
TCGAGGAAACATTGTACCTGCTGCAGTCGCCGGCGAACGCGCGCGCGCTGATCGAATCGATCGCCGAGCTGGACGCCGGC
AAGGGTAGCGAGCGCGACCTGATCGCGCCGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.68 Da
• Isoelectric Point: 8.0670

>AT215445 WP_010948599.1 NZ_CP082847:791900-792190 [Legionella pneumophila]
MDKKLFERLYESMTQMNEIIDGERVPSREFVVDAVHVKEIRKKTGLTQEKFCHLIDVSIGTLRNWEQGRREPTGPAKALL
RAINKDPEHVLAALAG

Nucleotide

• Length: 291 bp

>AT215445 NZ_CP110678:2168766-2169032 [Sphingomonas sp. S1-29]
GTGAAGCTGGTCTTTTCGTCGCGCGCCTGGGACCAGTATCTCGAATGGCAGGGCGACGATCGGCGCGGGCTGGAGCGCAT
CAACGCATTGCTCAAGGAATGCATGCGCGATCCCTTTCGCGGAACCGGCAAGCCCGAGCCGCTGGGCGGCAATCTAGCCG
GCTGGTGGTCGCGCCGGATCACCCGCGAGGATCGGCTGGTGTATCGGGTTACGGGTAAGGGTGAGGGGCAGGTGCTGGAA
GTGGCGCAGTGTCGGTATCATTATTGA

Structures

Toxin

• AlphaFold DB: Q5ZRG7