Detailed information of TA system TA021544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 305534..306240
• Replicon: plasmid 2
• Accession: NC_007950
• Organism: Polaromonas sp. JS666

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: Q11Z97
• Locus tag: BPRO_RS27730
• Protein ID: WP_011486373.1
• Coordinates: 305534..305836 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: Q11Z96
• Locus tag: BPRO_RS27735
• Protein ID: WP_011486374.1
• Coordinates: 305839..306240 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BPRO_RS27715	300848..301429	+	582	WP_081430613.1	DUF4158 domain-containing protein	-
BPRO_RS27720	301333..302655	-	1323	WP_011486371.1	IS110 family transposase	-
BPRO_RS27725	302917..305454	+	2538	WP_011486372.1	Tn3 family transposase	-
BPRO_RS27730	305534..305836	+	303	WP_011486373.1	type II toxin-antitoxin system MqsR family toxin	Toxin
BPRO_RS27735	305839..306240	+	402	WP_011486374.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
BPRO_RS27740	306204..307670	-	1467	WP_157046091.1	tyrosine-type recombinase/integrase	-
BPRO_RS27745	307811..308743	-	933	WP_011486376.1	DNA-binding protein	-
BPRO_RS27750	309289..310365	+	1077	WP_011486377.1	alkene reductase	-
BPRO_RS27755	310411..311022	+	612	WP_011486378.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..338007	338007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-99)

Antitoxin

• Antitoxin MqsA/HigA-2 (1-131)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10928.63 Da
• Isoelectric Point: 6.8580

>T21544 WP_011486373.1 NC_007950:305534-305836 [Polaromonas sp. JS666]
MEKGTPHCKLPVVQALIEAGRVQATASAFGGARDLGINDLTGMCAVVMSLTFANFYKSMTTHADHRIWQDVYHAKTANSA
DVYLKLTVIDDVLIVSFKEL

Nucleotide

• Length: 303 bp

>T21544 NC_007950:305534-305836 [Polaromonas sp. JS666]
ATGGAAAAAGGCACTCCTCACTGCAAGCTGCCAGTCGTTCAAGCCTTGATCGAAGCCGGGAGGGTCCAAGCAACGGCAAG
CGCCTTCGGCGGTGCTCGTGACTTGGGTATCAATGACCTGACTGGGATGTGCGCTGTTGTCATGTCGCTCACGTTCGCCA
ACTTCTACAAGAGCATGACGACCCATGCCGATCACCGGATTTGGCAAGACGTGTACCACGCCAAGACAGCCAACAGTGCC
GATGTGTACTTGAAGCTAACCGTCATTGATGACGTTTTAATTGTTTCTTTCAAGGAGCTGTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14833.00 Da
• Isoelectric Point: 7.1650

>AT21544 WP_011486374.1 NC_007950:305839-306240 [Polaromonas sp. JS666]
MKCPCCGAAELIHDTRDMPYTYKGESTTIPAVTGDFCPACGEIVLNREHGDRYSEMLGQFQRQVNAAYVDPGYIATMRKR
LDLDQRQAAEIFGGGVNAFSRYENGKTKPPLALVKLLKVLERHPDLLNEVRVA

Nucleotide

• Length: 402 bp

>AT21544 NC_007950:305839-306240 [Polaromonas sp. JS666]
ATGAAATGCCCATGCTGCGGTGCCGCAGAACTGATCCACGACACCCGCGATATGCCCTATACCTACAAGGGCGAATCCAC
CACCATCCCAGCTGTGACAGGCGACTTTTGCCCGGCTTGCGGTGAGATTGTATTGAACCGTGAACACGGTGACCGCTACA
GCGAAATGCTGGGCCAGTTTCAGCGCCAGGTGAACGCAGCCTATGTCGATCCCGGCTACATTGCCACCATGCGTAAGCGG
CTTGACCTAGACCAGCGTCAGGCCGCTGAAATATTCGGTGGAGGGGTCAATGCCTTTTCTCGTTACGAGAACGGCAAGAC
CAAGCCACCATTGGCCCTCGTCAAGCTGCTCAAGGTGCTTGAGCGTCACCCAGATCTGCTCAACGAAGTCAGAGTCGCCT
GA

Structures

Toxin

• AlphaFold DB: Q11Z97

Antitoxin

• AlphaFold DB: Q11Z96