Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 356784..357430 Replicon chromosome
Accession NZ_CP082840
Organism Klebsiella pneumoniae strain W09308

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag K6041_RS01645 Protein ID WP_002920560.1
Coordinates 356784..357131 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag K6041_RS01650 Protein ID WP_002920557.1
Coordinates 357131..357430 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6041_RS01635 (K6041_01635) 352710..354143 + 1434 WP_002920564.1 glycogen synthase GlgA -
K6041_RS01640 (K6041_01640) 354161..356608 + 2448 WP_002920561.1 glycogen phosphorylase -
K6041_RS01645 (K6041_01645) 356784..357131 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6041_RS01650 (K6041_01650) 357131..357430 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
K6041_RS01655 (K6041_01655) 357493..359001 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
K6041_RS01660 (K6041_01660) 359206..359535 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
K6041_RS01665 (K6041_01665) 359586..360416 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
K6041_RS01670 (K6041_01670) 360466..361224 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T215404 WP_002920560.1 NZ_CP082840:356784-357131 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T215404 NZ_CP110674:c1722060-1721671 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCGCGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT215404 WP_002920557.1 NZ_CP082840:357131-357430 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT215404 NZ_CP110674:c1722284-1722057 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References