Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 327650..328344 Replicon chromosome
Accession NZ_CP082835
Organism Escherichia coli strain 1613

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag K8O78_RS01515 Protein ID WP_001263495.1
Coordinates 327946..328344 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag K8O78_RS01510 Protein ID WP_000554757.1
Coordinates 327650..327943 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8O78_RS01490 (323361) 323361..323858 + 498 Protein_289 REP-associated tyrosine transposase RayT -
K8O78_RS01495 (324003) 324003..325715 - 1713 Protein_290 flagellar biosynthesis protein FlhA -
K8O78_RS01500 (325687) 325687..326472 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
K8O78_RS01505 (326543) 326543..327598 + 1056 WP_001226186.1 DNA polymerase IV -
K8O78_RS01510 (327650) 327650..327943 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
K8O78_RS01515 (327946) 327946..328344 + 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
K8O78_RS01520 (328354) 328354..328806 + 453 WP_001059871.1 GNAT family N-acetyltransferase -
K8O78_RS01525 (329125) 329125..329328 + 204 Protein_296 RtcB family protein -
K8O78_RS01530 (329327) 329327..329848 + 522 Protein_297 peptide chain release factor H -
K8O78_RS01535 (329905) 329905..331362 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
K8O78_RS01540 (331623) 331623..332081 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (332677) 332677..332757 + 81 NuclAT_11 - -
- (332677) 332677..332757 + 81 NuclAT_11 - -
- (332677) 332677..332757 + 81 NuclAT_11 - -
- (332677) 332677..332757 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T215362 WP_001263495.1 NZ_CP082835:327946-328344 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T215362 NZ_CP110661:c2368357-2368255 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT215362 WP_000554757.1 NZ_CP082835:327650-327943 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT215362 NZ_CP110661:2368248-2368393 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References