Detailed information of TA system TA021535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4359076..4359678
• Replicon: chromosome
• Accession: NC_007946
• Organism: Escherichia coli UTI89

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: UTI89_RS21625
• Protein ID: WP_000897302.1
• Coordinates: 4359076..4359387 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: UTI89_RS21630
• Protein ID: WP_000356397.1
• Coordinates: 4359388..4359678 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UTI89_RS21600	4354990..4355589	+	600	WP_001314324.1	glucose-1-phosphatase	-
UTI89_RS21605	4355583..4356455	+	873	WP_000920762.1	virulence factor BrkB family protein	-
UTI89_RS21610	4356452..4356889	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
UTI89_RS21615	4356934..4357875	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
UTI89_RS21620	4357939..4358847	-	909	WP_001331553.1	alpha/beta hydrolase	-
UTI89_RS21625	4359076..4359387	+	312	WP_000897302.1	hypothetical protein	Toxin
UTI89_RS21630	4359388..4359678	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
UTI89_RS21640	4360037..4360315	+	279	WP_001296612.1	hypothetical protein	-
UTI89_RS21645	4360711..4360929	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
UTI89_RS21650	4361145..4362074	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
UTI89_RS21655	4362071..4362706	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
UTI89_RS21660	4362703..4363605	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T21535 WP_000897302.1 NC_007946:4359076-4359387 [Escherichia coli UTI89]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T21535 NC_007946:4359076-4359387 [Escherichia coli UTI89]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT21535 WP_000356397.1 NC_007946:4359388-4359678 [Escherichia coli UTI89]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT21535 NC_007946:4359388-4359678 [Escherichia coli UTI89]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416