Detailed information of TA system TA215332

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83881..84135
• Replicon: plasmid unnamed
• Accession: NZ_CP082832
• Organism: Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K8B75_RS27285
• Protein ID: WP_001312851.1
• Coordinates: 83986..84135 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	83881..83942 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B75_RS27250 (79533)	79533..79745	+	213	WP_005012601.1	hypothetical protein	-
K8B75_RS27255 (80046)	80046..80135	-	90	Protein_103	IS1 family transposase	-
K8B75_RS27260 (80190)	80190..80867	+	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
K8B75_RS27265 (80867)	80867..81214	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
K8B75_RS27270 (81234)	81234..82805	+	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
K8B75_RS27275 (82843)	82843..83459	-	617	Protein_107	IS1-like element IS1A family transposase	-
K8B75_RS27280 (83560)	83560..83742	+	183	WP_000968309.1	hypothetical protein	-
- (83881)	83881..83942	-	62	NuclAT_4	-	Antitoxin
- (83881)	83881..83942	-	62	NuclAT_4	-	Antitoxin
- (83881)	83881..83942	-	62	NuclAT_4	-	Antitoxin
- (83881)	83881..83942	-	62	NuclAT_4	-	Antitoxin
K8B75_RS27285 (83986)	83986..84135	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
K8B75_RS27290 (84419)	84419..84676	+	258	WP_000083833.1	replication regulatory protein RepA	-
K8B75_RS27295 (84912)	84912..84986	+	75	WP_032336874.1	RepA leader peptide Tap	-
K8B75_RS27300 (84979)	84979..85425	+	447	Protein_112	plasmid replication initiator RepA	-
K8B75_RS27305 (85425)	85425..86039	-	615	Protein_113	VENN motif pre-toxin domain-containing protein	-
K8B75_RS27310 (86746)	86746..87966	+	1221	WP_000410951.1	arginine deiminase	-
K8B75_RS27315 (87977)	87977..88888	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / mph(A) / sul1 / qacE / aadA5	senB	1..186443	186443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T215332 WP_001312851.1 NZ_CP082832:83986-84135 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T215332 NZ_CP110645:c2356319-2355393 [Aeromonas salmonicida]
ATGAATAAAGACTCCGTCTATTACCGACAGGTAGCATTACTGCTCAAGATGCTGCCAATCATCGCCAATGAGAAGATGTT
TGCCCTAAAAGGGGGAACTGCTATCAATCTTTTTGTACGTGACTTCCCCCGACTGTCTGTTGATATCGACTTATCCTATC
TGCCACTAGAGCCTCGAGAAGAAGCGATAGCCAATGTCAGGGCCGCATTGCAGCGCATTGTCGAACAAGCGAATAGCCAG
AAAGGCCTACAAGCCCGTCTTCAAGACAATAACGGGGATGAACTCAGGATTATCGTCACAGGCGCAGCGACGATCAAAAT
CGAACTCTCGCCAGTTGCCCGAGGCACACTACATGACCCCGAGGTGCTTCCTGTGGTTGAAGATGTCGAAACTGAGTTTG
GCTACGCCGAAATAGCAGTTGTCAGCATGCCTGACCTTTACGGGGGCAAACTCTGCGCTGCAATGGACAGACAACACCCA
CGGGATCTGTTTGACGTCCGAATGCTATTGGCTGCAGAGGGGATAACCCGGGAGATATTCGTGGGCTTCCTCGCTTACAC
ACTGGGCCATCCCAGACCTATCAGCGAAGTGATGGACCCTAGATGGAAAGATCTCTCTGACGCGTTTCAACAGGAGTTTG
AAGGAATGACTAACGAGCCTGTCACCTTGGAGGAGCTGCTTGAAGTCCCCGAAGCAATGTTTGCCGCTTTAAAAGCCCAG
TTTATCCCACGCGATAAAGACTTTCTCCTATCCTTCAAACGAGGAGAGCCTGACTGGACACTGTTTGATGTACCTACCGC
AGAGCAATTACCCGCGATTCGATGGAAACTACAGAATTTGCAAAAGCTACGAGACAAGAACCCAGAAAAACATCAAGAGG
CATTGGTAAAACTCGATAATGTTTTAACGACATGGCTTGTTAAATAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT215332 NZ_CP082832:c83942-83881 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin