Detailed information of TA system TA215329

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43827..44060
• Replicon: plasmid unnamed
• Accession: NZ_CP082832
• Organism: Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: K8B75_RS27055
• Protein ID: WP_001323520.1
• Coordinates: 43944..44060 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	43827..43858 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B75_RS27020 (39948)	39948..40445	+	498	WP_042039995.1	single-stranded DNA-binding protein	-
K8B75_RS27025 (40513)	40513..40746	+	234	WP_000005990.1	DUF905 family protein	-
K8B75_RS27030 (40774)	40774..40971	+	198	Protein_54	hypothetical protein	-
K8B75_RS27035 (41026)	41026..41460	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
K8B75_RS27040 (41457)	41457..42219	+	763	Protein_56	plasmid SOS inhibition protein A	-
K8B75_RS27045 (42188)	42188..42376	-	189	WP_001299721.1	hypothetical protein	-
- (42188)	42188..42385	+	198	NuclAT_0	-	-
- (42188)	42188..42385	+	198	NuclAT_0	-	-
- (42188)	42188..42385	+	198	NuclAT_0	-	-
- (42188)	42188..42385	+	198	NuclAT_0	-	-
K8B75_RS27050 (42433)	42433..43802	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
- (43827)	43827..43858	+	32	NuclAT_3	-	Antitoxin
- (43827)	43827..43858	+	32	NuclAT_3	-	Antitoxin
- (43827)	43827..43858	+	32	NuclAT_3	-	Antitoxin
- (43827)	43827..43858	+	32	NuclAT_3	-	Antitoxin
K8B75_RS28320 (43844)	43844..43993	+	150	Protein_59	plasmid maintenance protein Mok	-
K8B75_RS27055 (43944)	43944..44060	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
K8B75_RS28325 (44280)	44280..44510	+	231	WP_071886920.1	hypothetical protein	-
K8B75_RS28330 (44508)	44508..44680	-	173	Protein_62	hypothetical protein	-
K8B75_RS28335 (44750)	44750..44956	+	207	WP_000547968.1	hypothetical protein	-
K8B75_RS27060 (44981)	44981..45268	+	288	WP_000107535.1	hypothetical protein	-
K8B75_RS27065 (45386)	45386..46207	+	822	WP_001234426.1	DUF932 domain-containing protein	-
K8B75_RS27070 (46504)	46504..47106	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
K8B75_RS27075 (47427)	47427..47810	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K8B75_RS27080 (47997)	47997..48686	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / mph(A) / sul1 / qacE / aadA5	senB	1..186443	186443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T215329 WP_001323520.1 NZ_CP082832:43944-44060 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T215329 NZ_CP110638:c5370190-5369807 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 32 bp

>AT215329 NZ_CP082832:43827-43858 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin