Detailed information of TA system TA215327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5239157..5239759
• Replicon: chromosome
• Accession: NZ_CP082831
• Organism: Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: K8B75_RS25760
• Protein ID: WP_000897302.1
• Coordinates: 5239448..5239759 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8B75_RS25755
• Protein ID: WP_000356397.1
• Coordinates: 5239157..5239447 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B75_RS25720 (5234464)	5234464..5235393	+	930	WP_223065280.1	formate dehydrogenase accessory protein FdhE	-
K8B75_RS25725 (5235575)	5235575..5235817	-	243	WP_001068514.1	CopG family transcriptional regulator	-
K8B75_RS25730 (5236107)	5236107..5236955	+	849	WP_001038650.1	hypothetical protein	-
K8B75_RS28280 (5236980)	5236980..5237720	+	741	WP_000608806.1	hypothetical protein	-
K8B75_RS25740 (5237905)	5237905..5238123	-	219	WP_001251293.1	CopG family transcriptional regulator	-
K8B75_RS25745 (5238520)	5238520..5238798	-	279	WP_001296612.1	hypothetical protein	-
K8B75_RS25750 (5238860)	5238860..5239072	-	213	WP_000197774.1	hypothetical protein	-
K8B75_RS25755 (5239157)	5239157..5239447	-	291	WP_000356397.1	NadS family protein	Antitoxin
K8B75_RS25760 (5239448)	5239448..5239759	-	312	WP_000897302.1	hypothetical protein	Toxin
K8B75_RS25765 (5239988)	5239988..5240896	+	909	WP_001385591.1	alpha/beta hydrolase	-
K8B75_RS25770 (5240960)	5240960..5241901	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
K8B75_RS25775 (5241946)	5241946..5242383	-	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
K8B75_RS25780 (5242380)	5242380..5243252	-	873	WP_000920762.1	virulence factor BrkB family protein	-
K8B75_RS25785 (5243246)	5243246..5243845	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T215327 WP_000897302.1 NZ_CP082831:c5239759-5239448 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T215327 NZ_CP110638:c4889802-4889518 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT215327 WP_000356397.1 NZ_CP082831:c5239447-5239157 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT215327 NZ_CP110638:c4890034-4889792 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCTGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: S1P416