Detailed information of TA system TA215308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1884979..1885481
• Replicon: chromosome
• Accession: NZ_CP082831
• Organism: Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: K8B75_RS08855
• Protein ID: WP_000767819.1
• Coordinates: 1885227..1885481 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: K8B75_RS08850
• Protein ID: WP_001259253.1
• Coordinates: 1884979..1885230 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B75_RS08825 (1880157)	1880157..1881224	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
K8B75_RS08830 (1881224)	1881224..1882294	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
K8B75_RS08835 (1882291)	1882291..1883595	-	1305	WP_001296211.1	histidinol dehydrogenase	-
K8B75_RS08840 (1883601)	1883601..1884500	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
K8B75_RS08845 (1884646)	1884646..1884696	-	51	WP_001364200.1	his operon leader peptide	-
K8B75_RS08850 (1884979)	1884979..1885230	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
K8B75_RS08855 (1885227)	1885227..1885481	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
K8B75_RS08860 (1885564)	1885564..1886388	+	825	WP_000754737.1	SDR family oxidoreductase	-
K8B75_RS08865 (1886434)	1886434..1887363	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
K8B75_RS08870 (1887578)	1887578..1887640	+	63	WP_010723108.1	membrane protein YoeI	-
K8B75_RS08875 (1887630)	1887630..1888988	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
K8B75_RS08880 (1889205)	1889205..1889663	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1889205..1889663	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T215308 WP_000767819.1 NZ_CP082831:1885227-1885481 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T215308 NZ_CP110634:572021-572371 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT215308 WP_001259253.1 NZ_CP082831:1884979-1885230 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT215308 NZ_CP110634:571735-572016 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3