Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 640597..641324 Replicon chromosome
Accession NZ_CP082831
Organism Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag K8B75_RS03150 Protein ID WP_000550189.1
Coordinates 640597..640911 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K8B75_RS03155 Protein ID WP_000560269.1
Coordinates 640908..641324 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B75_RS03130 (636754) 636754..637740 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
K8B75_RS03135 (637819) 637819..638511 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
K8B75_RS03140 (638588) 638588..639091 - 504 WP_001295542.1 M48 family metallopeptidase -
K8B75_RS03145 (639176) 639176..640312 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
K8B75_RS03150 (640597) 640597..640911 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
K8B75_RS03155 (640908) 640908..641324 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
K8B75_RS03160 (641369) 641369..643387 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
K8B75_RS03165 (643613) 643613..645964 - 2352 WP_000695432.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T215305 WP_000550189.1 NZ_CP082831:640597-640911 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T215305 NZ_CP110623:c2705082-2704801 [Prosthecochloris sp. SCSIO W1101]
ATGAGGACGATTAAGTACACATCAAAGTTCAAAAGCGATTACAAGCGGGAGCAAAAAGGGCGTCATGGAAAAAAGCTGGA
ACGTCTATTGAAACCAGTCATCAGCCTACTGGTCGAAGATCAGGCGTTGCCGAAAAACCTTGTTGATCACCCATTGAGCG
GCCGATGGAAGGATTGCCGAGATTGTCACATAAAGCCTGATCTTGTGCTCATATATCGAAAACCCGATGACCAAAATCTT
GAACTGGTGCGTCTTGGTTCACATAGCGAGTTGGGACTATAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT215305 WP_000560269.1 NZ_CP082831:640908-641324 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT215305 NZ_CP110623:c2705332-2705069 [Prosthecochloris sp. SCSIO W1101]
ATGATTACAAAATCTGTTGTGCGGGCAAGGATTGATCAGGAAACCAAGGATGAAGCTTCGGCAATTCTTGCTGCTATGGG
GCTGACGATGTCAGATGCTGTAAGGTTACTGATGAAAAAAATCGTAGCGGAGAAAGCGTTGCCGTTTAATCCGCTGATTC
CGAATAAGGAAACAATTCAGGCGATCAAAGAGTCGAGAGCAGGCGAGACGCATACTGCCGGATCGGTAGATGAGCTATTC
AAAGAACTCGATGAGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References