Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 600070..600869 Replicon chromosome
Accession NZ_CP082831
Organism Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag K8B75_RS02940 Protein ID WP_000347251.1
Coordinates 600070..600534 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag K8B75_RS02945 Protein ID WP_001296435.1
Coordinates 600534..600869 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B75_RS02910 (595071) 595071..595505 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K8B75_RS02915 (595523) 595523..596401 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K8B75_RS02920 (596391) 596391..597170 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K8B75_RS02925 (597181) 597181..597654 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K8B75_RS02930 (597677) 597677..598957 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
K8B75_RS02935 (599206) 599206..600015 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K8B75_RS02940 (600070) 600070..600534 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K8B75_RS02945 (600534) 600534..600869 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K8B75_RS02950 (601018) 601018..602589 - 1572 WP_001273763.1 galactarate dehydratase -
K8B75_RS02955 (602964) 602964..604298 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
K8B75_RS02960 (604314) 604314..605084 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T215304 WP_000347251.1 NZ_CP082831:c600534-600070 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T215304 NZ_CP110622:2175489-2175797 [Prosthecochloris sp. SCSIO W1103]
GTGAATAGCGAGTTTGCGACATTCGACATAGCCGATTATCTGGATAACGAAGAGGTCATTACCGAATATCTTTCCGCTGC
AACGGAAGATCCGAATCCTGATGTTTTTATTGCCGCTCTCGGTGACGTTGCTAAAGCCCGAGGCATGACACAGATCGCCA
AAGATTCGGGTTTAGGCAGGGAGAGTTTATATAAAGCGTTAAGCGCAGGATCACATCCACGATTTGAAACAATCAACGCT
GTCCTTCATGCTCTTGGTGTAAAGATTGCCATCATTCCAGCCGATAGGAGACAGAAAAAGACTTCGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT215304 WP_001296435.1 NZ_CP082831:c600869-600534 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT215304 NZ_CP110622:2175187-2175492 [Prosthecochloris sp. SCSIO W1103]
ATGAATACTTTTATTCGATCAAACGAATTTGACCGATGGCTTTCCAACCTTTCAGACCAGAAAGCAAAAGCAAGAATCCT
GGCAAGACTTCGGAGTGCGATGCATGGCAATTTCGGTGATTGTGAGCCTGTCGGCGAAGGCGTATCGGAAATGCGCATCC
ATGTCGGAGCAGGTTATCGAGTATACTACACTCGAACAGGAACTACCGTTTACTTCCTGCTAGGCGGGGGCAGCAAGTCA
ACTCAGCGAAAAGATATCAAGAACCTAAAAAAACTAGCCAGGAAAATCAAGGAGACAGAACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References