Detailed information of TA system TA215297

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31162..31426
• Replicon: plasmid unnamed2
• Accession: NZ_CP082829
• Organism: Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: K8B73_RS25645
• Protein ID: WP_001387489.1
• Coordinates: 31162..31314 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	31366..31426 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B73_RS25615 (26428)	26428..28596	+	2169	WP_023518847.1	DotA/TraY family protein	-
K8B73_RS25620 (28667)	28667..29329	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
K8B73_RS25625 (29401)	29401..29610	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
K8B73_RS25630 (30002)	30002..30178	+	177	WP_001054897.1	hypothetical protein	-
K8B73_RS25635 (30243)	30243..30539	-	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
K8B73_RS25640 (30839)	30839..31090	+	252	WP_001291964.1	hypothetical protein	-
K8B73_RS25645 (31162)	31162..31314	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (31366)	31366..31426	+	61	NuclAT_0	-	Antitoxin
- (31366)	31366..31426	+	61	NuclAT_0	-	Antitoxin
- (31366)	31366..31426	+	61	NuclAT_0	-	Antitoxin
- (31366)	31366..31426	+	61	NuclAT_0	-	Antitoxin
K8B73_RS25650 (31635)	31635..32720	+	1086	WP_074446708.1	protein finQ	-
- (32790)	32790..32847	+	58	NuclAT_1	-	-
- (32790)	32790..32847	+	58	NuclAT_1	-	-
- (32790)	32790..32847	+	58	NuclAT_1	-	-
- (32790)	32790..32847	+	58	NuclAT_1	-	-
K8B73_RS25655 (33027)	33027..34235	+	1209	WP_011264049.1	IncI1-type conjugal transfer protein TrbA	-
K8B73_RS25660 (34254)	34254..35324	+	1071	WP_011264050.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..106249	106249

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T215297 WP_001387489.1 NZ_CP082829:c31314-31162 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T215297 NZ_CP110620:1874073-1874399 [Pasteurella multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAATG
ATTTGCGAAGTCCACCAGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAATATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT215297 NZ_CP082829:31366-31426 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin