Detailed information of TA system TA215290

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 4387418..4387830
• Replicon: chromosome
• Accession: NZ_CP082827
• Organism: Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: K8B73_RS21145
• Protein ID: WP_000132601.1
• Coordinates: 4387489..4387830 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4387418..4387494 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B73_RS21135 (4384281)	4384281..4385870	+	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-
K8B73_RS21140 (4385867)	4385867..4387261	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
- (4387418)	4387418..4387494	-	77	NuclAT_12	-	Antitoxin
- (4387418)	4387418..4387494	-	77	NuclAT_12	-	Antitoxin
- (4387418)	4387418..4387494	-	77	NuclAT_12	-	Antitoxin
- (4387418)	4387418..4387494	-	77	NuclAT_12	-	Antitoxin
- (4387418)	4387418..4387494	-	77	NuclAT_13	-	Antitoxin
- (4387418)	4387418..4387494	-	77	NuclAT_13	-	Antitoxin
- (4387418)	4387418..4387494	-	77	NuclAT_13	-	Antitoxin
- (4387418)	4387418..4387494	-	77	NuclAT_13	-	Antitoxin
K8B73_RS21145 (4387489)	4387489..4387830	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
K8B73_RS21150 (4387992)	4387992..4389371	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
K8B73_RS21155 (4389371)	4389371..4390417	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T215290 WP_000132601.1 NZ_CP082827:4387489-4387830 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T215290 NZ_CP110619:3776750-3777007 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT215290 NZ_CP082827:c4387494-4387418 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin