Detailed information of TA system TA215287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3995051..3995701
• Replicon: chromosome
• Accession: NZ_CP082827
• Organism: Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K8B73_RS19330
• Protein ID: WP_000263532.1
• Coordinates: 3995360..3995701 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: K8B73_RS19325
• Protein ID: WP_000212552.1
• Coordinates: 3995051..3995350 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B73_RS19300 (3990499)	3990499..3990810	-	312	WP_000415004.1	hypothetical protein	-
K8B73_RS19305 (3990815)	3990815..3991207	-	393	WP_000285322.1	flagellar export chaperone FliS	-
K8B73_RS19310 (3991230)	3991230..3992546	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
K8B73_RS19315 (3992751)	3992751..3993665	-	915	WP_000949083.1	lateral flagellin LafA	-
K8B73_RS19320 (3994151)	3994151..3994993	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
K8B73_RS19325 (3995051)	3995051..3995350	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
K8B73_RS19330 (3995360)	3995360..3995701	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8B73_RS19335 (3995777)	3995777..3996754	-	978	WP_001195938.1	hypothetical protein	-
K8B73_RS19340 (3996771)	3996771..3997700	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
K8B73_RS19345 (3997715)	3997715..3999091	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
K8B73_RS19350 (3999267)	3999267..3999566	-	300	WP_000867281.1	rod-binding protein	-
K8B73_RS19355 (3999566)	3999566..4000666	-	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T215287 WP_000263532.1 NZ_CP082827:c3995701-3995360 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T215287 NZ_CP110619:3187021-3187464 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT215287 WP_000212552.1 NZ_CP082827:c3995350-3995051 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT215287 NZ_CP110619:3186777-3187034 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7