Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2580987..2581549 Replicon chromosome
Accession NZ_CP082827
Organism Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag K8B73_RS12250 Protein ID WP_000605676.1
Coordinates 2580987..2581265 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag K8B73_RS12255 Protein ID WP_000781370.1
Coordinates 2581265..2581549 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B73_RS12225 (2576573) 2576573..2577004 - 432 WP_000152305.1 peroxiredoxin OsmC -
K8B73_RS12230 (2577349) 2577349..2577564 + 216 WP_000495766.1 biofilm-dependent modulation protein -
K8B73_RS12235 (2577666) 2577666..2577803 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
K8B73_RS12240 (2577960) 2577960..2579657 + 1698 WP_223107116.1 malate dehydrogenase -
K8B73_RS12245 (2579791) 2579791..2580801 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
K8B73_RS12250 (2580987) 2580987..2581265 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8B73_RS12255 (2581265) 2581265..2581549 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
K8B73_RS12260 (2581777) 2581777..2582430 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
K8B73_RS12265 (2582423) 2582423..2583307 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
K8B73_RS12270 (2583320) 2583320..2586367 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T215282 WP_000605676.1 NZ_CP082827:2580987-2581265 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T215282 NZ_CP110619:c3074909-3074514 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT215282 WP_000781370.1 NZ_CP082827:2581265-2581549 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT215282 NZ_CP110619:c3074488-3074222 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References