Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 634814..635613 Replicon chromosome
Accession NZ_CP082827
Organism Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag K8B73_RS03065 Protein ID WP_000347269.1
Coordinates 634814..635278 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag K8B73_RS03070 Protein ID WP_001309780.1
Coordinates 635278..635613 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B73_RS03035 (629815) 629815..630249 - 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K8B73_RS03040 (630267) 630267..631145 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K8B73_RS03045 (631135) 631135..631914 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K8B73_RS03050 (631925) 631925..632398 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K8B73_RS03055 (632421) 632421..633701 - 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
K8B73_RS03060 (633950) 633950..634759 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
K8B73_RS03065 (634814) 634814..635278 - 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K8B73_RS03070 (635278) 635278..635613 - 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K8B73_RS03075 (635762) 635762..637333 - 1572 WP_001273738.1 galactarate dehydratase -
K8B73_RS03080 (637708) 637708..639042 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
K8B73_RS03085 (639058) 639058..639828 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T215267 WP_000347269.1 NZ_CP082827:c635278-634814 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T215267 NZ_CP110619:c2227405-2226986 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT215267 WP_001309780.1 NZ_CP082827:c635613-635278 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT215267 NZ_CP110619:c2227674-2227414 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References